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Development of Mirror-Image VHHs with Less Immunogenicity / by Keisuke Aoki.
Springer eBooks EBA - Springer Chemistry and Material Science Collection 2025 Available online
View online- Format:
- Book
- Author/Creator:
- Aoki, Keisuke.
- Series:
- Springer Theses, Recognizing Outstanding Ph.D. Research, 2190-5061
- Language:
- English
- Subjects (All):
- Chemistry.
- Pharmacy.
- Proteins.
- Immunogenetics.
- Chemical Synthesis.
- Local Subjects:
- Chemical Synthesis.
- Pharmacy.
- Proteins.
- Immunogenetics.
- Physical Description:
- 1 online resource (140 pages)
- Edition:
- 1st ed. 2025.
- Place of Publication:
- Singapore : Springer Nature Singapore : Imprint: Springer, 2025.
- Summary:
- This book outlines the chemical synthesis of therapeutic targets and a screening process for unexplored mirror-image single-domain antibodies (D-VHH). This book first describes the chemical synthesis of model VHH and the characteristics of both enantiomeric VHHs, including binding activity, biodistribution and immunogenicity. Immunogenicity testing in mice demonstrated that administration of conventional L-VHH induced the generation of anti-drug antibodies while D-VHH-binding antibodies were not observed in D-VHH-immunized mice. Second, it is explained that the T7-phage-based mirror-image screening system was developed to identify D-VHH antibody fragments capable of binding to vascular endothelial growth factors. Additionally, this book details the chemical synthesis of full-length hepatitis B virus core protein and interleukin-6 for the application of exploring D-VHHs and other mirror-image molecules. Demonstrating that it is possible to develop a D-VHH that preferentially binds the native target protein and exhibits reduced immunogenicity, this book is useful for anyone interested in developing alternative biotherapeutics with reduced immunogenicity by combining chemical and biological approaches.
- Contents:
- Chapter 1.Introduction
- Chapter 2.Chemical Synthesis of Mirror-Image VHHs and Evaluation of Their Immunogenicity
- Chapter 3.Identification of a Mirror-Image VHH against Vascular Endothelial Growth Factor
- Chapter 4.Synthetic Study of Full-Length Hepatitis B Virus Core Protein and Its Capsid Assembly
- Chapter 5.Chemical Synthesis of Interleukin-6 for Mirror-Image VHH Discovery
- Chapter 6.Conclusions.
- Notes:
- Description based on publisher supplied metadata and other sources.
- ISBN:
- 9789819613014
- 9819613019
- OCLC:
- 1484883055
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