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Synthesis and Characterization of 1) Novel Fluorescent Natural and Unnatural Amino Acids, and 2) Tetrazine Derivatives / Robert John Micikas.

Dissertations & Theses @ University of Pennsylvania Available online

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Format:
Book
Thesis/Dissertation
Author/Creator:
Micikas, Robert John, author.
Contributor:
University of Pennsylvania. Chemistry, degree granting institution.
Language:
English
Subjects (All):
Chemistry.
Analytical chemistry.
Organic chemistry.
Chemistry--Penn dissertations.
Penn dissertations--Chemistry.
Local Subjects:
Chemistry.
Analytical chemistry.
Organic chemistry.
Chemistry--Penn dissertations.
Penn dissertations--Chemistry.
Physical Description:
1 online resource (229 pages)
Distribution:
Ann Arbor : ProQuest Dissertations & Theses, 2023
Contained In:
Dissertations Abstracts International 84-12B.
Place of Publication:
[Philadelphia, Pennsylvania] : University of Pennsylvania, 2022.
Language Note:
English
Summary:
Fluorescent amino acids (FAAs) offer significant advantages over fluorescent proteins in applications where the fluorophore size needs to be limited or minimized. A long-sought goal in biological spectroscopy/microcopy is to develop visible FAAs by modifying the indole ring of tryptophan. In Chapter 1 of this thesis, I discuss the investigation of the absorption spectra of a library of 4-substituted indoles and find that the frequency of the absorption maximum correlates linearly with the global electrophilicity index of the substituent. In Chapter 2, a scalable synthesis of Fmoc-protected blue fluorescent amino acid, L-4-cyanotryptophan (W4CN), exploiting an enantioselective phase-transfer catalyzed alkylation is presented. Finally, in Chapter 3, Large-Scale Synthesis and Assessment of the Potential Thermal Hazards of 3,6-dichlorotetrazine (DCT) will be discussed.
Notes:
Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
Advisors: Smith, Amos B., III; Committee members: Winkler, Jeffery D.; Percec, Virgil; Joullie, Madeleine M.
Department: Chemistry.
Ph.D. University of Pennsylvania 2023.
Local Notes:
School code: 0175
ISBN:
9798379754310
Access Restriction:
Restricted for use by site license.

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