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The Art of Chemistry: Tools for Chemical Biology and Conservation Science / Rachel Mara Lackner.
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Lackner, Rachel Mara, author.
- Language:
- English
- Subjects (All):
- Chemistry.
- Chemistry--Penn dissertations.
- Penn dissertations--Chemistry.
- Local Subjects:
- Chemistry.
- Chemistry--Penn dissertations.
- Penn dissertations--Chemistry.
- Physical Description:
- 1 online resource (374 pages)
- Contained In:
- Dissertations Abstracts International 84-08B.
- Place of Publication:
- [Philadelphia, Pennsylvania] : University of Pennsylvania, 2022.
- Ann Arbor : ProQuest Dissertations & Theses, 2022
- Language Note:
- English
- Summary:
- Chemical tools, which enable highly specific, selective observation of chemical orbiological processes at a molecular level, are extremely powerful resources for thebuilding of knowledge across interconnected research fields. A synthetic approachenables fine-tuning of a probe's activity and allows visualization of a mechanism orinteraction at a molecular level. These tools can be used as complements to analyticaltechniques such as microscopy and spectroscopy to gain information regarding achemical process, interaction, or material. The best-known application of these tools is tochemical biology, but fields outside of the traditional academic environment, such asmuseum conservation science, benefit from a creative synthetic approach as well. Thisthesis will explore several applications of novel chemical tools, from fluorescent dyes, totools for controlling protein-protein interactions, to complex metal substrates for surfaceenhanced Raman spectroscopy (SERS). The first part of this thesis will discuss thesynthesis of tools for chemically induced dimerization (CID) of proteins and thedevelopment of a quantitative, microscopy-based assay for the evaluation andoptimization of linker structure for heterobifunctional chemical tools. Next, the synthesisof fluorogenic, pH sensing dyes and preliminary work developing a photocleavableprotecting group (PPG) based on the dimethylamino quinoline scaffold will be presented.Finally, pivoting from chemical biology to conservation science, the use of gold raspberrymetamolecules (RMMs) for on-fiber SERS analysis of organic dyes will be discussed.
- Notes:
- Source: Dissertations Abstracts International, Volume: 84-08, Section: B.
- Advisors: Chenoweth, David M.; Committee members: Petersson, E. James; Kozlowski, Marisa C.; Molander, Gary A.
- Department: Chemistry.
- Ph.D. University of Pennsylvania 2022.
- Local Notes:
- School code: 0175
- ISBN:
- 9798374413120
- Access Restriction:
- Restricted for use by site license.
- This item is not available from ProQuest Dissertations & Theses.
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