Signal Transduction Immunohistochemistry : Methods and Protocols / edited by Alexander E. Kalyuzhny.

Format:

Book

Contributor:

Kalyuzhny, Alexander E., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2593

Methods in Molecular Biology, 1940-6029 ; 2593

Language:

English

Subjects (All):

Biology-Technique.

Experimental immunology.

Tissues.

Chemistry.

Immunological Techniques.

Local Subjects:

Immunological Techniques.

Tissues.

Physical Description:

1 online resource (XI, 328 pages) : 84 illustrations, 67 illustrations in color.

Edition:

3rd edition 2023.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2023.

System Details:

text file PDF

Summary:

This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.

Contents:

Top 5 Considerations When Choosing an Antibody

Validating Antibody Specificities for Immunohistochemistry by Protein Blotting

Recent Advances in Chromogens for Immunohistochemistry

Overview of Imaging Techniques in Immunohistochemistry

Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells

Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments

Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining

Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species

3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes

Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research

Protocol and Software for Automated Detection of Lysosome Active "Runs" and "Flights" with Wavelet Transform Approach

Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing

In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells

In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue

Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry

Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells

Immunohistochemical Techniques for Phosphoproteins

Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells

Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain Sections

Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry

Highly Multiplexed Immuno-Fluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometryTM.

Other Format:

Printed edition:

ISBN:

978-1-0716-2811-9

9781071628119

Access Restriction:

Restricted for use by site license.