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Vaccine Delivery Technology : Methods and Protocols / edited by Blaine A. Pfeifer, Andrew Hill.
- Format:
- Book
- Series:
- Springer Protocols (Springer-12345)
- Methods in molecular biology 1940-6029 ; 2183
- Methods in Molecular Biology, 1940-6029 ; 2183
- Language:
- English
- Subjects (All):
- Chemistry.
- Biotechnology.
- Biomaterials.
- Immunology.
- Biology-Technique.
- Pharmacology.
- Biological Techniques.
- Local Subjects:
- Chemistry.
- Biotechnology.
- Biomaterials.
- Immunology.
- Biological Techniques.
- Pharmacology.
- Physical Description:
- 1 online resource (XV, 590 pages) : 149 illustrations, 127 illustrations in color.
- Edition:
- 1st ed. 2021.
- Contained In:
- Springer Nature eBook
- Place of Publication:
- New York, NY : Springer US : Imprint: Humana, 2021.
- System Details:
- text file PDF
- Summary:
- This volume discusses the vaccine development process and the role delivery concepts contribute to a global goal of effective health outcomes. The chapters in this book cover a wide range of topics such as antigen discovery methods; genetic and protein antigen preparation; preparation of viral vaccines as VLPs; viral and non-viral gene delivery; needle-less or non-invasive delivery technology; vaccine storage; and vaccine administration and assessment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics; lists of the necessary materials and reagents; step-by-step, readily reproducible laboratory protocols; and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vaccine Delivery Technology: Methods and Protocols is a valuable resource for both novice and expert researchers, in and outside the field, who would like to gain insight into the impactful field of vaccines. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
- Contents:
- Vaccine Delivery and Immune Response Basics
- Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes
- Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies
- Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed
- Antigen Discovery in Bacterial Pan-Proteomes
- Alphavirus-Based Antigen Preparation
- Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells
- Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System
- High-Throughput Process Development for the Chromatographic Purification of Viral Antigens
- Production of Zika Virus Virus-Like Particles
- Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs)
- Up- and Downstream Processes for Viral Nanoplaxes as Vaccines
- Whole-Cell Vaccine Preparation: Options and Perspectives
- O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein
- Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines
- Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines
- Attenuation Methods for Live Vaccines
- Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism
- Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells
- Lipofection-Based Delivery of DNA Vaccines
- Flavivirus DNA Vaccine Design and Adjuvant Selection
- Vaccine Delivery with a Detoxified Bacterial Toxin
- Needle-Less or Non-Invasive Delivery Technology
- Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications
- A Hybrid Biological-Biomaterial Vector for Antigen Delivery
- Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens
- Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder
- The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers
- Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice
- The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot
- The Measurement of Antigen-Specific IgG Titers by Direct ELISA
- Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model
- Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils
- Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.
- Other Format:
- Printed edition:
- ISBN:
- 978-1-0716-0795-4
- 9781071607954
- Access Restriction:
- Restricted for use by site license.
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