Mass Spectrometry of Glycoproteins : Methods and Protocols / edited by Arnaud Delobel.

Format:

Book

Contributor:

Delobel, Arnaud, Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2271

Methods in Molecular Biology, 1940-6029 ; 2271

Language:

English

Subjects (All):

Mass spectrometry.

Chemistry.

Mass Spectrometry.

Local Subjects:

Mass Spectrometry.

Chemistry.

Physical Description:

1 online resource (XIV, 401 pages) : 140 illustrations, 126 illustrations in color.

Edition:

1st ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This volume presents methods used for the analysis of glycoproteins at different levels-intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.

Contents:

Glycosylation of Therapeutic Proteins: A Critical Quality Attribute

Characterization of Protein Glycoforms at Intact Level by Orbitrap Mass Spectrometry

Analysis of Intact Glycoproteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Fc Glycosylation Characterization of Human Immunoglobulins G using Immunocapture and LC-MS

Fast Afucosylation Profiling of Glyco-Engineered Antibody Subunits by Middle Up Mass Spectrometry

Characterization of Glycosylated Proteins at Subunit Level by HILIC/MS

Analysis of Monoclonal Antibody Glycopeptides by Capillary Electrophoresis - Mass Spectrometry Coupling (CE-MS)

Enrichment of Intact Glycopeptides using Strong Anion Exchange and Electrostatic Repulsion Hydrophilic Interaction Chromatography

In-Depth Glycan Characterization of Therapeutic Glycoproteins by Stepwise PGC -SPE and LC-MS/MS

Site-Specific N-glycosylation Analysis of Recombinant Proteins by LC/MSE

Mapping O-glycosylation Sites using OpeRATOR and LC-MS

Site-Specific O-Glycosylation Analysis by Liquid Chromatography/Mass Spectrometry with Electron-Transfer/Higher-Energy Collisional Dissociation

Profiling of N-Linked Oligosaccharides of a Glycoprotein by UPLC-FLR-ESI-MS after Derivatization with Fluorescent Anthranilamide

Evaluating N-glycosylation of a Therapeutic Monoclonal Antibody using UHPLC-FLR-MS with RapiFluor-MS Labeling

Routine Analysis of N-glycans using Liquid Chromatography Coupled to Routine Mass Detection

Online 2D-LC for Complex N-Glycan Analysis from Biopharmaceuticals

Profiling, Relative Quantification, and Identification of Sialylated N-Linked Oligosaccharides by UPLC-FLR-ESI/MS after Derivatization with Fluorescent Anthranilamide

Linkage Analysis of Oligo- and Polysaccharides: A Tutorial

Use of Exoglycosidases for the Structural Characterization of Glycans

Determination of Isomeric Glycan Structures by Permethylation and Liquid Chromatography-Mass Spectrometry (LC-MS)

Optimization of Multiple Glycosidase and Chemical Stabilization Strategies for N-glycan Isomer Detection by Mass Spectrometry Imaging in Formalin-Fixed, Paraffin-Embedded Tissues

Glycosylation Profiling of Glycoproteins Secreted from Cultured Cells Using Glycan Node Analysis and GC-MS

Array-Based N-Glycan Profiling of Cells in Culture

Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multi-Angle Light Scattering

Analysis of Glycoproteins by ATR-FTIR Spectroscopy: Comparative Assessment

Deploying Mass Spectrometric Data Analysis in the Amazon AWS Cloud Computing Environment.

Other Format:

Printed edition:

ISBN:

978-1-0716-1241-5

9781071612415

Access Restriction:

Restricted for use by site license.