Structural Proteomics : High-Throughput Methods / edited by Raymond J. Owens.

Format:

Book

Contributor:

Owens, Raymond J., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2305

Methods in Molecular Biology, 1940-6029 ; 2305

Language:

English

Subjects (All):

Life sciences.

Chemistry.

Life Sciences.

Local Subjects:

Life Sciences.

Chemistry.

Physical Description:

1 online resource (XII, 344 pages) : 88 illustrations, 67 illustrations in color.

Edition:

3rd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science. Chapter 9 is available open access under a CC BY 4.0 license.

Contents:

The Protein Data Bank Archive

Computational Methods for the Elucidation of Protein Structure and Interactions

Methods for Molecular Modeling of Protein Complexes

High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus

Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies

Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering

Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification

Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems

Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources

From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example

Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods

Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection

Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology

Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED.

Other Format:

Printed edition:

ISBN:

978-1-0716-1406-8

9781071614068

Access Restriction:

Restricted for use by site license.