G Protein-Coupled Receptor Screening Assays : Methods and Protocols / edited by Sofia Aires M. Martins, Duarte Miguel F. Prazeres.

Format:

Book

Contributor:

Martins, Sofia Aires M., Editor.

Prazeres, Duarte Miguel F., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2268

Methods in Molecular Biology, 1940-6029 ; 2268

Language:

English

Subjects (All):

Biological transport.

Cell membranes.

Pharmacology.

Chemistry.

Membrane Trafficking.

Local Subjects:

Membrane Trafficking.

Pharmacology.

Chemistry.

Physical Description:

1 online resource (XVI, 327 pages) : 78 illustrations, 69 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This fully updated edition targets not only those assays directly involved in the discovery of GPCR-active compounds but also those involved in cell-based experiments designed to study physiological responses. Whether coming from academia or industry, or being an experienced researcher or a newcomer to the field, the reader will find accessible methods and protocols that cover the latest developments on receptor purification, molecular biology, recombinant engineering, and analytical techniques that enable the real time monitoring of the complex GPCR signaling cascade and identification of potential drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, G Protein-Coupled Receptor Screening Assays: Methods and Protocols, Second Edition aims to provide the tools necessary to contribute to the advancement of GPCR research and discovery and ultimately lead to the availability of innovative and more efficient drugs.

Contents:

Isolation of Lipid Rafts by the Detergent and Non-Detergent Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy

Detection of GPCR mRNA Expression in Primary Cells via qPCR, Microarrays, and RNA-Sequencing

Construction of Recombinant Cell Lines for GPCR Expression

Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor

Screening for Serotonin Receptor 4 Agonists Using GPCR-Based Sensor in Yeast

Immobilization of Olfactory Receptors Carried by Nanosomes onto a Gold Sensor Surface

Screening Methods for Cell-Free Synthesized GPCR/Nanoparticle Samples

Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs: The Case of Cy3B-Labelled Ligands Binding to MC4 Receptors in Budded Baculoviruses

Bioluminescence in G Protein-Coupled Receptors Drug Screening Using Nanoluciferase and Halo-Tag Technology

Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction

Imaging of Genetically-Encoded FRET-Based Biosensors to Detect GPCR Activity

cAMP Biosensor Assay Using BacMam Expression System: Studying the Downstream Signaling of LH/hCG Receptor Activation

FLIPR Calcium Mobilization Assays in GPCR Drug Discovery

Live Cell Imaging and Optogenetics-Based Assays for GPCR Activity

Split-Tobacco Etch Virus (Split-TEV) Method in G Protein-Coupled Receptor Interacting Proteins

NanoLuc-Based Methods to Measure β-Arrestin2 Recruitment to G Protein-Coupled Receptors

Luciferase Complementation Approaches to Measure GPCR Signaling Kinetics and Bias

Gradient Tracking by Yeast GPCRs in a Microfluidics Chamber

Monitoring Intracellular Calcium in Response to GPCR Activation: Comparison Between Microtiter Plates and Microfluidic Assays

Homology Modeling Using GPCRM Web Service.

Other Format:

Printed edition:

ISBN:

978-1-0716-1221-7

9781071612217

Access Restriction:

Restricted for use by site license.