RNA Scaffolds : Methods and Protocols / edited by Luc Ponchon.

Format:

Book

Contributor:

Ponchon, Luc., Editor.

SpringerLink (Online service)

Series:

Springer Protocols (Springer-12345)

Methods in molecular biology 1940-6029 ; 2323

Methods in Molecular Biology, 1940-6029 ; 2323

Language:

English

Subjects (All):

Genetics.

Chemistry.

Genetics and Genomics.

Local Subjects:

Genetics and Genomics.

Chemistry.

Physical Description:

1 online resource (XIII, 288 pages) : 61 illustrations, 41 illustrations in color.

Edition:

2nd ed. 2021.

Contained In:

Springer Nature eBook

Place of Publication:

New York, NY : Springer US : Imprint: Humana, 2021.

System Details:

text file PDF

Summary:

This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools. These latest methods are used in the fields of molecular biology, cellular biology, nanotechnology, and structural biology. The chapters in this book cover topics such as RNA footprinting using small chemical reagents; using tRNA scaffolds to assist RNA crystallization; identification of RNA-binding proteins associated to RNA structural elements; and synthetic biology medicine and bacteria-based cancer therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Scaffolds: Methods and Protocols, Second Edition is a valuable resource that explores the fascinating uses of RNA in these different areas, and will inspire experts and novice researchers to further study this developing field. .

Contents:

Predicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA

RNA Footprinting Using Small Chemical Reagents

Improving RNA Crystal Diffraction Quality by Post-Crystallization Treatment

Using tRNA Scaffold to Assist RNA Crystallization

RNA Modeling with the Computational Energy Landscape Framework

Co-Expression and Co-Purification of RNA-Protein Complexes in Escherichia coli

In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold

Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds

Identification of RNA-Binding Proteins Associated to RNA Structural Elements

Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors

Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging

Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria

FRET-Analysis of RNA-Protein-Interactions using Spinach-Aptamers

Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach

Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells

Folding RNA-Protein Complex into Designed Nanostructures

An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA

Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs

Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics.

Other Format:

Printed edition:

ISBN:

978-1-0716-1499-0

9781071614990

Access Restriction:

Restricted for use by site license.