Biochemistry / by J. Stenesh.

Format:

Book

Author/Creator:

Stenesh, J., Author.

Language:

English

Subjects (All):

Biochemistry.

Evolution (Biology).

Chemistry.

Evolutionary Biology.

Local Subjects:

Biochemistry.

Evolutionary Biology.

Chemistry.

Physical Description:

1 online resource (XXVII, 568 p. 1405 illus., 1327 illus. in color.)

Edition:

1st ed. 1998.

Place of Publication:

New York, NY : Springer US : Imprint: Springer, 1998.

Language Note:

English

Summary:

This text is intended for an introductory course in bio­ metabolism concludes with photosynthesis. The last sec­ chemistry. While such a course draws students from vari­ tion of the book, Part IV, TRANSFER OF GENETIC INFOR­ ous curricula, all students are presumed to have had at MATION, also opens with an introductory chapter and then least general chemistry and one semester of organic chem­ explores the expression of genetic information. Replica­ istry. tion, transcription, and translation are covered in this or­ My main goal in writing this book was to provide stu­ der. To allow for varying student backgrounds and for pos­ sible needed refreshers, a number of topics are included as dents with a basic body of biochemical knowledge and a thorough exposition of fundamental biochemical con­ four appendixes. These cover acid-base calculations, principles of cepts, including full definitions of key terms. My aim has of organic chemistry, tools biochemistry, and been to present this material in a reasonably balanced oxidation-reduction reactions. form by neither deluging central topics with excessive de­ Each chapter includes a summary, a list of selected tail nor slighting secondary topics by extreme brevity. readings, and a comprehensive study section that consists Every author of an introductory text struggles with of three types of review questions and a large number of the problem of what to include in the coverage. My guide­ problems.

Contents:

I. Foundation of Biochemistry

1. Frameworks of Biochemistry

II. Biomolecules

2. Amino Acids and Peptides

3. Proteins

4. Enzymes

5. Carbohydrates

6. Lipids and Membranes

7. Nucleic Acids

III. Metabolism

8. Introduction to Metabolism

9. Bioenergetics

10. Carbohydrate Metabolism

11. The Citric Acid Cycle

12. Electron Transport and Oxidative Phosphorylation

13. Lipid Metabolism

14 Amino Acid and Nucleotide Metabolism

15. Photosynthesis

IV. Transfer of Genetic Information

16. Introduction to Molecular Biology

17. Replication—The Synthesis of DNA

18. Transcription—The Synthesis of RNA

19. Translation—The Synthesis of Protein

Appendix A. Acid—Base Calculations

A.1. Ionic Strength

A.2. pH

A.3. Henderson—Hasselbalch Equation

A.4. Buffers

Appendix B. Principles of Organic Chemistry

B.1. Functional Groups

B.2. Polar Reactions

B.3. Chirality

B.4. Optical Isomerism

B.5. Conformation and Configuration

Appendix C. Tools of Biochemistry

C.1. Spectrophotometry

C.2. Chromatography

C.2.1. Adsorption Chromatography

C.2.2. Ion-Exchange Chromatography

C.2.3. Partition Chromatography

C.2.4. Gel-Filtration Chromatography

C.3. Centrifugation

C3.1. Analytical Ultracentrifugation

C.3.2. Density Gradient Centrifugation

C.4. Electrophoresis

C.5. Radioactivity

C.6. Recombinant DNA Technology

C.6.1. Selection of Target DNA Fragment

C.6.2. Production of Recombinant DNA

C.6.3. Insertion of Recombinant DNA into Host Cells

C.6.4. Selection of Cells Containing Cloned DNA

C.6.5. Polymerase Chain Reaction

Appendix D. Oxidation—Reduction Reactions

D.1. Half-Reactions

D.2. Direction of Redox Reactions

D.2.1. Standard Conditions

D.2.2. Actual Conditions

Answers to Problems.

Notes:

Bibliographic Level Mode of Issuance: Monograph

Includes bibliographical references and index.

ISBN:

1-4757-9427-4