Mapping small molecule binding sites with phage display technology / Lee Makowski.

Format:

Video

Author/Creator:

Makowski, Lee, speaker, author.

Series:

Henry Stewart talks. Biomedical & life sciences collection. Chemical biology : the role of chemistry in our fundamental understanding of biology.

Chemical biology : the role of chemistry in our fundamental understanding of biology, 2056-452X

Language:

English

Subjects (All):

Biology.

Chemistry.

Medical Subjects:

Biology.

Chemistry.

Genre:

Video recordings.

Physical Description:

1 online resource (1 streaming video file (39 min.)) : color, sound.

polychrome

Place of Publication:

London : Henry Stewart Talks, 2008.

System Details:

Mode of access: World Wide Web.

video file

Contents:

Contents: How do you identify the molecular target of a small molecule drug?

Flexible regions are key players

P-loop of ATP-binding proteins is an important example

Can display libraries of flexible peptides on proteins select those that bind to the ligand

Selection is through an affinity process called biopanning

Success depends on the diversity of the library

Diversity depends on how many different peptides are in the library and their relative abundance

RELIC can be used to calculate the diversity of a library from the sequences of ~100 peptides

Identification of motifs from a population is easy if the motif is very strong

Taxol-binding peptides were also identified by phage display and those peptides were used to identify the position on tubulin where taxol is bound and to identify Bcl-2 as a taxolbinding protein

Significant data from a variety of sources argue that the taxol-Bcl-2 interaction is important to the clinical efficacy of taxol.

Notes:

Animated audio-visual presentation with synchronized narration.

Title from title frames.

Publisher Number:

1717 Henry Stewart Talks

Access Restriction:

Restricted for use by site license.