Protein Sequencing Protocols / edited by Bryan John Smith.

Format:

Book

Contributor:

Smith, Bryan John, editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 64.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 64

Language:

English

Subjects (All):

Chemistry.

Analytical chemistry.

Analytical Chemistry.

Local Subjects:

Chemistry.

Analytical Chemistry.

Physical Description:

1 online resource (XIV, 375 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 1997.

System Details:

text file PDF

Summary:

Bryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using automated sequencers and/or mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acid by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. They also deal with such troublesome problems as identifying sites of modification and handling very small samples. Protein Sequencing Protocols offers self-contained, step-by-step recipes that ensure easily reproducible results for those already doing sequencing, and for those who are still learning the methods, as well as for those already in one area but wishing to learn associated techniques. Its multiplicity of proven protocols will guide the challenged experimenter to successful results.

Contents:

Strategies for Handling Polypeptides on a Microscale

SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing

Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization

Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization

Enzymatic Cleavage of Polypeptides

Chemical Cleavage of Polypeptides

Preparation of Glycopeptides

Selective Isolation of the Carboxy Terminal Peptide from a Protein

Selective Isolation of the Amino-Terminal Peptide from ?-Amino Blocked Protein

Analytical and Micropreparative Capillary Electrophoresis of Peptides

Reverse-Phase HPLC Purification of Peptides from Natural Sources for Structural Analysis

High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues

Hydrolysis of Samples for Amino Acid Analysis

Amino Acid Analysis

Postcolumn Amino Acid Analysis

Fast-Atom Bombardment and Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins

Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry

N-Terminal Protein Ladder Sequencing

The Dansyl-Edman Method for Manual Peptide Sequencing

The Dansyl Method for Identifying N-Terminal Amino Acids

Identification of PTH-Amino Acids by High-Performance Liquid Chromatography

Protein Sequencer Maintenance and Troubleshooting

Identifying the Carboxy Terminal Amino Acid of a Protein

Enzymatic and Chemical Methods for Manual C-Terminal Peptide Sequencing

Automated Methods for C-Terminal Protein Sequencing

Chemical Modification of Proteins for Sequence Analysis

On-Membrane Deblocking of Proteins

Analysis of Sites of Protein Phosphorylation

Identification of Sites of Glycosylation

Quantification and Location of Disulfide Bonds in Proteins.

Other Format:

Printed edition:

ISBN:

9781592595501

Access Restriction:

Restricted for use by site license.