Downstream Processing of Proteins : Methods and Protocols / edited by Mohamed A. Desai.

Format:

Book

Contributor:

Desai, Mohamed A., editor.

SpringerLink (Online service)

Series:

Methods in biotechnology 1940-6061 ; 9.

Springer Protocols (Springer-12345)

Methods in Biotechnology, 1940-6061 ; 9

Language:

English

Subjects (All):

Chemistry.

Biotechnology.

Local Subjects:

Chemistry.

Biotechnology.

Physical Description:

1 online resource (IX, 229 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2000.

System Details:

text file PDF

Summary:

In Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.

Contents:

Downstream Processing in the Biotechnology Industry

2 Microbial Cell Disruption by High-Pressure Homogenization

3 Protein Concentration and Buffer Exchange Using Ultrafiltration

4 High-Performance Tangential Flow Filtration for Protein Separations

Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation

Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules

Application of Chromatography in the Downstream Processing of Biomolecules

Affinity Ligand Technology

Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules

Expanded Bed Adsorption in the Purification of Biomolecules

Application of HPLC in the Purification of Biomolecules

Countercurrent Chromatography of Proteins with Polymer Phase Systems

Practical Aspects and Applications of Radial Flow Chromatography

Sterile Filtration and Virus Filtration

Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products

Validation of Sterilizing Filters in the Biotechnology Industry.

Other Format:

Printed edition:

ISBN:

9781592590278

Access Restriction:

Restricted for use by site license.