Nanotechnology for Nucleic Acid Delivery : Methods and Protocols / edited by Manfred Ogris, David Oupicky.

Format:

Book

Contributor:

Ogris, Manfred, editor.

Oupicky, David, editor.

SpringerLink (Online service)

Series:

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 948.

Springer Protocols (Springer-12345)

Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 948

Language:

English

Subjects (All):

Chemistry.

Biotechnology.

Nanotechnology.

Local Subjects:

Chemistry.

Biotechnology.

Nanotechnology.

Physical Description:

1 online resource (XII, 286 pages) : 70 illustrations, 50 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.

System Details:

text file PDF

Summary:

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Contents:

Toxicological Aspects for Nanomaterial in Humans

Lactate Dehydrogenase Assasy for Assessment of Polycation Cytotoxicity

Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determincation for Assessment of Polycation-Mediated Cytotoxicity

Anti-PEG IgM Production Via a PEGylated Nano-Carrier System for Nucleic Acid Delivery

Near-Infrared Optical Imaging of Nucleic Acid Nanocarrier In Vivo

Lipids for Nucleic Acid Delivery: Synthesis and Particle Formantion

Histidine-Rich Cationic Amphipathic Peptides for Plasmid DNA and siRNA Delivery

Synthesis of Polyethylenimine Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

Synthesis of Bioreducible Polycations with Controlled Topologies

Lyophilization of Synthetic Gene Carriers

Surface-and Hydrogel-Mediated Delivert of Nucleic Acid Nanoparticles

Layer-by-Layer Assembled Gold Nanoparticles for the Delivery of Nucleic Acids

In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

Enhancing Nucleic Acid Delivery with Ultrasound and Microbubbles

Magnetic And Acoustically Active Microbubbles Loaded with Nucleic Acids for Gene Delivery

Synthesis of Lipidic Magnetic Nanoparticles for Nucleic Acid Delivery

Lipopeptide Delivery of siRNA to the Central Nervous System

Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery

Liver-Targeted Gene Delivery Through Retrograde Intrabiliary Infusion.

Other Format:

Printed edition:

ISBN:

9781627031400

Access Restriction:

Restricted for use by site license.