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Glycoanalysis Protocols / edited by Elizabeth F. Hounsell.

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Holman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
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Format:
Book
Contributor:
Hounsell, Elizabeth F., editor.
SpringerLink (Online service)
Series:
Methods in molecular biology 1064-3745 ; 76.
Springer Protocols (Springer-12345)
Methods in Molecular Biology™, 1064-3745 ; 76
Language:
English
Subjects (All):
Chemistry.
Analytical chemistry.
Analytical Chemistry.
Local Subjects:
Chemistry.
Analytical Chemistry.
Physical Description:
1 online resource (X, 262 pages).
Edition:
Second Edition.
Contained In:
Springer eBooks
Place of Publication:
Totowa, NJ : Humana Press, 1998.
System Details:
text file PDF
Summary:
Now fully updated and considerably expanded, Glycoanalysis Protocols, 2nd edition, makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd edition, continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.
Contents:
Characterization of Protein Glycosylation
Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides
Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography
Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM)
HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column
HPLC and HPAEC of Oligosaccharides and Glycopeptides
Analysis of N- and O-Glycans by Pyridylamination
Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins
Analysis of Glycosaminoglycans and Proteoglycans
The Use of Cell and Organ Culture for the Study of Secreted Mucins
Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides
TLC in Structure and Recognition Studies of Glycosphingolipids
Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids
Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures
Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique.
Other Format:
Printed edition:
ISBN:
9781592595624
Access Restriction:
Restricted for use by site license.

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