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Synthetic Biology / edited by Karen M. Polizzi, Cleo Kontoravdi.
Connect to full text Available online
View onlineHolman Biotech Commons QH506 .M45 v.1 (1984)-v.20 (1993),v.22 (1994),v.24 (1994)-v.53 (1996), v.42 (1995) and v.51 (1995) reported missing 3-13-2000 v.55 (1995),v.58 (1996)-v.63 (1997), v.65 (1996)-v.154 (2001), v.156 (2001)-190 (2002), v.192 (2002)-v.407 (2007) v.409 (2007)-v.416 (2008),v.418 (2008)-v.466 v.468-v.490,v.492,v.494,v.496-499 501-506,508,510-512,514,516-517,519-536 538,540-569,571 573-589,591-608,610-615,617,620-627,630-633,636,638,642
Available
- Format:
- Book
- Series:
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1073.
- Springer Protocols (Springer-12345)
- Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1073
- Language:
- English
- Subjects (All):
- Chemistry.
- Biotechnology.
- Biochemical engineering.
- Biochemical Engineering.
- Local Subjects:
- Chemistry.
- Biotechnology.
- Biochemical Engineering.
- Physical Description:
- 1 online resource (X, 226 pages) : 80 illustrations, 48 illustrations in color.
- Contained In:
- Springer eBooks
- Place of Publication:
- Totowa, NJ : Humana Press : Imprint: Humana Press, 2013.
- System Details:
- text file PDF
- Summary:
- In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology. Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.
- Contents:
- What is Synthetic Biology?
- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
- BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit
- Overlap Extension PCR Cloning
- One-Step Isothermal Assembly of DNA Fragments
- Creation and Characterization of Component Libraries for Synthetic Biology
- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements
- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli
- Construction and Engineering of Large Biochemical Pathways via DNA Assembler
- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors
- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research
- Combinatorial DNA Assembly using Golden Gate Cloning
- Construction of Synthetic Gene Circuits in the Escherichia coli Genome
- Shuffling of DNA Cassettes in a Synthetic Integrin
- Systematic Methodology for the Development of Mathematical Models for Biological Processes
- SOBOLHDMR: A General Purpose Modelling Software.
- Other Format:
- Printed edition:
- ISBN:
- 9781627036252
- Access Restriction:
- Restricted for use by site license.
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