Biosensors and Biodetection : Methods and Protocols: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors / edited by Avraham Rasooly, Keith E. Herold.

Format:

Book

Contributor:

Rasooly, Avraham, editor.

Herold, K. E., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 504.

Springer Protocols (Springer-12345)

Methods in Molecular Biology™, 1064-3745 ; 504

Language:

English

Subjects (All):

Chemistry.

Biotechnology.

Radiology.

Imaging / Radiology.

Local Subjects:

Chemistry.

Biotechnology.

Imaging / Radiology.

Physical Description:

1 online resource (XVIII, 464 pages).

Contained In:

Springer eBooks

Place of Publication:

Totowa, NJ : Humana Press, 2009.

System Details:

text file PDF

Summary:

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors focuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

Contents:

Mechanical Detectors

A Set of Piezoelectric Biosensors Using Cholinesterases

Piezoelectric Biosensors for Aptamer-Protein Interaction

Piezoelectric Quartz Crystal Resonators Applied for Immunosensing and Affinity Interaction Studies

Biosensors Based on Cantilevers

Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors

Electrochemical Detectors

Preparation of Screen-Printed Electrochemical Immunosensors for Estradiol, and Their Application in Biological Fluids

Electrochemical DNA Biosensors: Protocols for Intercalator-Based Detection of Hybridization in Solution and at the Surface

Electrochemical Biosensor Technology: Application to Pesticide Detection

Electrochemical Detection of DNA Hybridization Using Micro and Nanoparticles

Electrochemical Immunosensing Using Micro and Nanoparticles

Methods for the Preparation of Electrochemical Composite Biosensors Based on Gold Nanoparticles

Lateral Flow

Immunochromatographic Lateral Flow Strip Tests

Liposome-Enhanced Lateral-Flow Assays for the Sandwich-Hybridization Detection of RNA

Rapid Prototyping of Lateral Flow Assays

Lateral Flow Colloidal Gold-Based Immunoassay for Pesticide

Ligands

Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen

In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents

Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands

Biosensor Detection Systems: Engineering Stable, High-Affinity Bioreceptors by Yeast Surface Display

Antibody Affinity Optimization Using Yeast Cell Surface Display

Using RNA Aptamers and the Proximity Ligation Assay for the Detection of Cell Surface Antigens

In Vitro Selection of Protein-Binding DNA Aptamers as Ligands for Biosensing Applications

Protein and DNA Preparation

Immobilization of Biomolecules onto Silica and Silica-Based Surfaces for Use in Planar Array Biosensors

Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler.

Other Format:

Printed edition:

ISBN:

9781603275699

Access Restriction:

Restricted for use by site license.