High Content Screening : A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery / edited by Paul A. Johnston, Oscar J. Trask.

Format:

Book

Contributor:

Johnston, Paul A., editor.

Trask, Oscar J., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1683.

Methods in Molecular Biology, 1064-3745 ; 1683

Language:

English

Subjects (All):

Chemistry.

Biotechnology.

Local Subjects:

Chemistry.

Biotechnology.

Physical Description:

1 online resource (XIII, 397 pages) : 82 illustrations, 71 illustrations in color.

Edition:

Second edition 2018.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This second edition details some of the recent trends in HCS/HCA/HCI. New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field.

Contents:

Applications and Caveats on the Utilization of DNA-specific Probes in Cell-based Assays

General Staining and Segmentation Procedures for High Content Imaging and Analysis

Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis

Cell-based High Content Analysis of Cell Proliferation and Apoptosis

Tools to Measure Autophagy using High Content Imaging and Analysis

Guidelines for Microplate Selection in High Content Imaging

Quality Control for High-Throughput Imaging Experiments using Machine Learning in CellProfiler

High Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR and Small Molecule Screening

Strategies and solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays

Live-Cell High Content Screening in Drug Development

Challenges and Opportunities in Enabling High-Throughput, Miniaturized High-Content Screening

Translocation Biosensors - Versatile Tools to Probe Protein Functions In Living Cells

High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions

High Content Imaging Assays for IL-6-induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines

Single Cell and Population Level Analysis of HCA Data

Utilization of Multidimensional Data in the Analysis of Ultra High Throughput High Content Phenotypic Screens

High Content Screening of Mammalian Primary Cortical Neurons

Human Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications

Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High-Content Assays

The Generation of Three-Dimensional Head and Neck Cancer Models for Drug Discovery in 384-well Ultra-Low Attachment Microplates

An Endothelial Cell/Mesenchymal Stem Cell Co-Culture Cord Formation Assay to Model Vascular Biology In Vitro

High-throughput Automated Chemical Screens in Zebrafish.

Other Format:

Printed edition:

ISBN:

9781493973576

Access Restriction:

Restricted for use by site license.