Virus-Derived Nanoparticles for Advanced Technologies : Methods and Protocols / edited by Christina Wege, George P. Lomonossoff.

Format:

Book

Contributor:

Wege, Christina, editor.

Lomonossoff, George P., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1776.

Methods in Molecular Biology, 1064-3745 ; 1776

Language:

English

Subjects (All):

Chemistry.

Biotechnology.

Local Subjects:

Chemistry.

Biotechnology.

Physical Description:

1 online resource (XV, 671 pages) : 156 illustrations, 108 illustrations in color.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2018.

System Details:

text file PDF

Summary:

This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Virus-Derived Nanoparticles for Advanced Technologies: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

The Production in Plants of Mosaic Turnip Crinkle Virus-like Particles Derived by Co-infiltration of Wild-Type and Modified Forms of Virus Coat Protein

Isolation and Characterization of Two Distinct Types of Unmodified Spherical Plant Sobemovirus-like Particles for Diagnostic and Technical Uses

RNA-directed Assembly of Tobacco Mosaic Virus (TMV)-Like Carriers with Tunable Fractions of Differently Addressable Coat Proteins

Fabrication of Tobacco Mosaic Virus-like Nanorods for Peptide Display

In planta Production of Fluorescent Filamentous Plant Virus-based Nanoparticles

Self-assembling Plant-derived Vaccines against Papillomaviruses

Recombinant Expression of Tandem-HBc Virus-like Particles (VLPs)

Production and Application of Insect Virus-based VLPs

Nanomanufacture of Free-standing, Porous, Janus-Type Films of Polymer-Plant Virus Nanoparticle Arrays

Self-assembly of Rod-like Bionanoparticles at Interfaces and in Solution

Bottom-up Assembly of TMV-based Nucleoprotein Architectures on Solid Supports

Internal Deposition of Cobalt Metal and Iron Oxide within CPMV eVLPs

Plant Virus-based Nanoparticles for the Delivery of Agronomic Compounds as a Suspension Concentrate

Nanowires and Nanoparticle Chains inside Tubular Viral Template

In vitro-Reassembled Plant Virus-Like Particles of Hibiscus Chlorotic Ringspot Virus (HCRSV) as Nano-protein Cages for Drugs

CCMV-based Enzymatic Nanoreactors

Protocol for the Efficient Cell-free Synthesis of Cowpea Chlorotic Mottle Virus-like Particles Containing Heterologous RNAs

Packaging DNA Origami into Viral Protein Cages

In Vitro Assembly of Virus-Derived Designer Shells around Inorganic Nanoparticles

In Vivo Packaging of Protein Cargo inside of Virus-Like Particle P22

Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids

Delivering Cargo: Plant-based Production of Bluetongue Virus core- and Virus-like Particles Containing Fluorescent Proteins

Bioinspired Silica Mineralization on Viral Templates

Propagation and Isolation of Tobacco Mosaic Virus Which Surface Displays Metal Binding and Reducing Peptides for Generation of Gold Nanoparticles

TMV-templated Formation of Metal and Polymer Nanotubes

Semiconducting Hybrid Layer Fabrication Scaffolded by Virus Shells

Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits

Drug-loaded Plant-virus Based Nanoparticles for Cancer Drug Delivery

Construction of Artificial Enzymes on a Virus Surface

Redox-Immuno Functionalized Potyvirus Nanoparticles for High Resolution Imaging by AFM-SECM Correlative Microscopy

Presenting Peptides at the Surface of Potyviruses in planta

Engineering of M13 Bacteriophage for Development of Tissue Engineering Materials

Displaying Whole-chain Proteins on Hepatitis B virus Capsid-like Particles

Dual-functionalized Virus-gold Nanoparticles Clusters for Biosensing

TMV-based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications

Integrated Methods to Manufacture Hydrogel Microparticles Containing Viral-metal Nanocomplexes with High Catalytic Activity

Integrated Methods to Manufacture Hydrogel Microparticles with High Protein Conjugation Capacity and Binding Kinetics via Viral Nanotemplate Display

Interactions between Plant Viral Nanoparticles (VNPs) and Blood Plasma Proteins, and their Impact on the VNP In Vivo Fates

Fabrication of Plant Virus- based Thin Films to Modulate the Osteogenic Differentiation of Mesenchymal Stem Cells

Dual Surface Modification of Genome-free MS2 Capsids for Delivery Applications

Virus-Based Cancer Therapeutics for Targeted Photodynamic Therapy.

Other Format:

Printed edition:

ISBN:

9781493978083

Access Restriction:

Restricted for use by site license.