Biomedical Nanotechnology : Methods and Protocols / edited by Sarah Hurst Petrosko, Emily S. Day.

Format:

Book

Contributor:

Hurst Petrosko, Sarah, editor.

Day, Emily S., editor.

SpringerLink (Online service)

Series:

Methods in molecular biology 1064-3745 ; 1570.

Methods in Molecular Biology, 1064-3745 ; 1570

Language:

English

Subjects (All):

Chemistry.

Biotechnology.

Physical Description:

1 online resource (XIV, 341 pages) : 91 illustrations, 77 illustrations in color.

Edition:

Second edition 2017.

Contained In:

Springer eBooks

Place of Publication:

New York, NY : Springer New York : Imprint: Humana Press, 2017.

System Details:

text file PDF

Summary:

This second edition volume provides an overview of some of the types of nanostructures commonly used in nanobiomedicine. The chapters in this book discuss practical information on the synthesis and characterization of a variety of solution-phase and surface-bound nanomaterials, with examples of how they can be used in sensing, imaging, and therapeutics. Specific topics include the synthesis and characterization of molecule and biomolecule-functionalized nanoconjugates with gold, iron oxide, or polymeric cores; the development of biosensing, imaging, and therapeutic applications of multicomponent/multifunctional nanostructures; and the application of flow cytometry in nanobiomedicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.< Thorough and comprehensive, Biomedical Nanotechnology: Methods and Protocols, Second Edition is a useful resource for scientists and researchers at all levels who are interested in working in a new area of nanoscience and technology, or in expanding their knowledge base in their current field.

Contents:

Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces

Ligand Exchange and 1H NMR Quantification of Single- and Mixed-Moiety Thiolated Ligand Shells on Gold Nanoparticles

Nanoparticle Tracking Analysis for Determination of Hydrodynamic Diameter, Concentration, and Zeta Potential of Polyplex Nanoparticles

Magnetic Characterization of Iron Oxide Nanoparticles for Biomedical Applications

Preparation of Magnetic Nanoparticles for Biomedical Applications

Brain-Penetrating Nanoparticles for Analysis of the Brain Microenvironment

Volumetric Bar-Chart Chips for Biosensing

qFlow Cytometry-Based Receptoromic Screening: A High-Throughput Quantification Approach Informing Biomarker Selection and Nanosensor Development

Evaluating Nanoparticle Binding to Blood Compartment Immune Cells in High-Throughput with Flow Cytometry

A Gold@Polydopamine Core-Shell Nanoprobe for Long-Term Intracellular Detection of MicroRNAs in Differentiating Stem Cells

Antibody-Conjugated Single Quantum Dot Tracking of Membrane Neurotransmitter Transporters in Primary Neuronal Cultures

Spectroscopic Photoacoustic Imaging of Gold Nanorods

Dual Wavelength-Triggered Gold Nanorods for Anti-Cancer Treatment

Photolabile Self-Immolative DNA-Drug Nanostructures

Enzyme-Responsive Nanoparticles for the Treatment of Disease

NanoScript: A Versatile Nanoparticle-Based Synthetic Transcription Factor for Innovative Gene Manipulation

Glucose-Responsive Insulin Delivery by Microneedle-Array Patches Loaded with Hypoxia-Sensitive Vesicles

Electrospun Nanofiber Scaffolds and their Hydrogel Composites for the Engineering and Regeneration of Soft Tissues

Application of Hydrogel Template Strategy in Ocular Drug Delivery

High-Accuracy Determination of Cytotoxic Responses from Graphene Oxide Exposure using Imaging Flow Cytometry

Air-Liquid Interface Cell Exposures to Nanoparticle Aerosols

Returning to the Patent Landscapes for Nanotechnology: Assessing the Garden That It Has Grown Into. .

Other Format:

Printed edition:

ISBN:

9781493968404

Access Restriction:

Restricted for use by site license.