Reaction detection in liquid chromatography / edited by Ira S. Krull.

Format:

Book

Contributor:

Krull, Ira S., 1940-

Series:

Chromatographic science ; v. 34.

Chromatographic science ; v. 34

Language:

English

Subjects (All):

High performance liquid chromatography.

Chemical reactions.

Chromatography, High Pressure Liquid.

Medical Subjects:

Chromatography, High Pressure Liquid.

Physical Description:

x, 377 pages : illustrations ; 24 cm.

Place of Publication:

New York : M. Dekker, [1986]

Summary:

The first book to focus entirely on reactions for analyte detection and characterization, Reaction Detection in Liquid Chromatography depicts off- and on-line, pre- and post-column approaches that have been successfully used for many classes of compounds, both organic and inorganic, in high performance liquid chromatography. The book gives special attention to methods and instrumentation associated with post-column reaction detection, discussing theory, background, principles, and equations ... and also highlights major areas of reaction chemistry, such as immobilized (or solution) enzymatic reactions, homogenous solution chemistry, photochemical derivation, pairedion reagents, solid phase and solid supported reagents, and reactions for inorganic species. In addition, Reaction Detection in Liquid Chromatography details the efficiencies of the various reactions surveyed ... forecasts how the utility of each reaction is likely to be enhanced by new research ... and gives data that will allow the reader to reproduce reaction-detection approaches for new analytes and samples. Reaction Detection in Liquid Chromatography is essential reading for analytical, bioanalytical, quality control, and research and development chemists. It also comprises a fine reference for analysts involved in development and applications of liquid chromatography for specific qualitative and quantitative analyte identification; and in-house, professional seminars.

Contents:

1. Fundamentals of Reaction Detection Systems / Bernhard Lillig, Heinz Engelhardt 1

II. Instrumentation 3

III. Dispersion in Open Tubular Reactor Systems 8

IV. Dispersion in Packed-Bed Reactors 38

V. Comparison of the Different Reactor Types 46

2. Solution Chemistry for Post-Column Reactions / Timothy D. Schlabach, Robert Weinberger 63

II. Instrumentation 64

III. Post-Column Reagents 69

IV. Post-Column Applications 77

V. Recent Developments 107

VI. Future Developments 117

3. Post-Column Reaction Detection of Inorganic Species / Richard M. Cassidy, Brent D. Karcher 129

II. Design 132

III. Chemistry 145

IV. Applications 157

4. Enzyme Reaction Detectors in HPLC / Larry D. Bowers 195

II. Analytical Considerations in the Use of Enzymes 196

III. Tandem HPLC-Immobilized Enzyme Systems 207

IV. Applications 215

5. Solid-Phase Reaction Detectors for HPLC / Stephen T. Colgan, Ira S. Krull 227

II. Construction of Solid-Phase Reactors 234

III. Polymeric Solid-Phase Reactors 235

IV. Nonpolymeric Solid-Phase Reactors 247

V. Commercial Solid-Phase Reaction Systems 251

6. Ion Pairing as a Means of Detection in Liquid Chromatography / James F. Lawrence, Udo A. Th. Brinkman, Roland W. Frei 259

III. Prechromatographic Counterion Addition 262

IV. Postchromatographic Counterion Addition 269

7. Post-Column Photochemical Derivatizations for Improved Detection and Identification in HPLC / Ira S. Krull, William R. LaCourse 303

II. Post-Column Photochemical Derivatizations for Improved HPLC-UV and HPLC-FL Detection 307

III. Post-Column Photochemical Derivatizations for Improved HPLC-EC Detection (HPLC-hv-EC) 321

IV. The Photoconductivity Detector in HPLC (HPLC-PCD) 334

V. The Photoionization Detector in HPLC 336

VI. The Photoelectrochemical Detector in HPLC 340

VII. Conclusions and Recommendations for Future Research and Development 343.

Notes:

Includes bibliographies and indexes.

ISBN:

0824775791

OCLC:

13666576