Chikungunya virus methods and protocols edited by Claudia Filomatori, Eliana F. Castro

Format:

Book

Contributor:

Filomatori, Claudia, editor.

Castro, Eliana F., editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology 1940-6029

Language:

English

Subjects (All):

Chikungunya--Laboratory manuals.

Chikungunya.

Physical Description:

1 online resource

Place of Publication:

Springer US, 2027.

New York, NY Humana Press [2026]

Summary:

This volume presents laboratory protocols to study different aspects of CHIKV biology, virus RNA and protein functions, virus-host interactions, pathogenesis, immune responses, diagnostic techniques, structural biology, vector competence and surveillance, antiviral strategies and treatment. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice needed to ensure success in the lab. Cutting-edge and authoritative, Chikungunya Virus: Methods and Protocols provides specific lab procedures, techniques, and applications to prepare for future outbreaks of arboviral diseases

Contents:

Overview of chikungunya virus epidemiology, biology, and pathogenesis

Naked-eye visual loop-mediated isothermal amplification method for the rapid detection of chikungunya virus

RT-RPA assisted CRISPR/Cas12a based Isothermal Detection of Chikungunya virus

Quantification of Chikungunya Virus RNA by Real-Time RT-PCR Assay

Determination of Chikungunya Virus Infectious Titer Using Plaque Assays

Automated Counting of Chikungunya Virus–Formed Plaques

Production of Viral Particles from a Chikungunya Virus Infectious Clone

Molecular Cloning and Reverse Genetics

A Restriction-Free Cloning Approach for Molecular Engineering of Plasmids

Viral Protein Expression and Purification

PAGE-based measurements of nsP2 protease activity

Assessing Chikungunya Virus Protease Activity and Pharmacological Inhibition Using the acc-CHIK15-dnp Fluorogenic Peptide Substrate

Epitope tagging and coimmunoprecipitation to identify viral protein interactors

Plaque reduction neutralization test against chikungunya virus using liver homogenate

Screening of Antiviral Agents against CHIKV using reporter virus

In silico drug design of chikungunya antivirals: using consensus methods for virtual screening

Surface Plasmon Resonance-Based Analysis of Ligand Binding Kinetics in Chikungunya Virus Drug Discovery

Analysis of cell populations from arbovirus infected tissues by flow cytometry

Chikungunya virus infection in paraffin-embedded tissue: analysis of histological alterations and viral detection by immunohistochemistry

Collection and processing of adult mosquitoes (Diptera: Culicidae) for molecular studies of arbovirus infection

Assessing the Vector Competence for Chikungunya Virus in Mosquitoes

Global Genomic Surveillance

Nanopore Sequencing for Chikungunya Virus: Principles and Application

Workflow for Long-Read Amplicon Sequencing of Chikungunya Virus Using Oxford Nanopore Technology

Optimized Amplicon Strategy for Long-Read Sequencing of the Chikungunya Virus Genome

Protocol for detecting and sequencing chikungunya virus from field-collected mosquitoes

Notes:

Includes index

Online resource; title from PDF title page (SpringerLink, viewed September 22, 2026)

ISBN:

9781071654965

1071654969

OCLC:

1617436080

Access Restriction:

Restricted for use by site license