Immunophenotyping methods and protocols edited by Angélique Biancotto, Pradeep K. Dagur

Format:

Book

Contributor:

Biancotto, Angélique, editor.

Dagur, Pradeep K., editor.

Series:

Methods in molecular biology (Clifton, N.J.) 1940-6029

Methods in molecular biology 1940-6029

Language:

English

Subjects (All):

Immunophenotyping--Laboratory manuals.

Immunophenotyping.

Physical Description:

1 online resource

Edition:

Second edition

Place of Publication:

Springer US, 2027.

New York, NY Humana Press [2026]

Summary:

This new edition reflects the transformative developments in immunophenotyping research while maintaining a strong foundation in established methodologies essential for both research and clinical applications. Beginning with chapters involving fundamental immune cell populations, the book continues by exploring clinical applications in hematological malignancies and cellular therapies, as well as methodological advances and analytical frameworks essential for modern immunophenotyping. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Practical and up-to-date, Immunophenotyping: Methods and Protocols, Second Edition aims to provide researchers and clinicians with representative protocols spanning fundamental techniques to cutting-edge technologies

Contents:

Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T-Cells

Enumeration of Benign and Malignant Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis

Neutrophil Subset Immunophenotyping

Natural Killer Cells and Innate Lymphoid Cells Immunoprofiling

Immunophenotyping of Human Regulatory T-Cells from Peripheral Blood Mononuclear Cells

Immunophenotyping of Human B Lymphocytes from Peripheral Blood Mononuclear Cells

Immunophenotyping Studies in Non-Human Primate Models

Immunophenotypic Assessment of Natural Killer Cell Degranulation and Monocyte Function from Cryopreserved Peripheral Blood Mononuclear Cells

Immunophenotyping of Acute Myeloid Leukemia

Flow Cytometric Immunophenotyping of Acute Lymphoblastic Leukemia

Clinical Flow Cytometric Testing in Chronic Lymphocytic Leukemia

Measurable Residual Disease

Immunophenotyping of Cellular Therapy Products Against Oncogenic and Viral Targets

Guidelines for Gating Flow Cytometry Data for Immunological Assays

Quality Control of Immunophenotyping

High Immunophenotyping of Human PBMCs Using Lyophilized Antibodies

Immunophenotyping by Single Cell CITE-Seq

Immunophenotyping by Spatial Biology

High Dimensional Immunophenotyping of Human Leukocytes by Spectral Cytometry

Build Heatmaps of Cell Frequencies and Marker Intensities for Correct Interpretations

High-Dimensional Multimodal Data Integration for Immune-Driven Diseases: A Practical Guide

Notes:

Online resource; title from PDF title page (SpringerLink, viewed October 6, 2026)

ISBN:

9781071656075

1071656074

OCLC:

1619833135

Access Restriction:

Restricted for use by site license