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Ultrastructural Neuroscience Methods.

SpringerProtocols (1984- current) Available online

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Format:
Book
Contributor:
Marie-Ève Tremblay
Language:
English
Subjects (All):
Neurosciences--Laboratory manuals.
Neurosciences.
Neurons--Ultrastructure--Laboratory manuals.
Neurons.
Place of Publication:
Springer US, 2027.
Summary:
This volume explores the study of the brain ultrastructure using various methodological approaches from different perspectives. The chapters in this book cover topics such as biological sample preparation, transmission and scanning electron microscopy imaging, and cutting-edge qualitative and quantitative analysis. The chapters provide essential considerations and protocols for the study of animal models, human cellular models, and human post-mortem brain samples across contexts of health and disease. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and thorough, Ultrastructural Neuroscience Methods is a key resource for biomedical, translational, and clinical researchers interested in delving into the mysteries of the brain at a nanoscale resolution.
Contents:
Freeze-Fracture Electron Microscopy Methods for Evaluation of Nerve Cells and Synapses
How to Combine Light and Electron Microscopy to Detect Lipids
Immunoelectron Microscopy of Brain Receptors: Focus on G Protein-Coupled Receptors
An Alternative Chromogenic Immunostaining Method for Light and Electron Microscopy: A Demonstration Using NeuN Staining in Mouse Brain Samples
Mapping Mitochondrial Architecture in the Brain: Methods and Insights
Ultrastructure of Glial Cells in iPSC-Derived Unguided Neural Organoids from APOE4/4 Patients with Alzheimer's Disease
A Guide for Microglial Ultrastructural Analysis
Reproducible Quantification Method to Differentiate Dark vs Intermediate vs Typical Appearance of Immune Cells in Electron Microscopy A Priori, Including Dark Microglia
The Dynamic Side of Electron Microscopy: How to Use Quantitative Electron Microscopy to Portray the Gold Particle's Dance Style
Multimodal Imaging of Breast-to-Brain-Metastatic Lesions
SnapG: An Automated Tool for Myelin g-ratio Measurement with Batch Processing Capability
Ultrastructural Volumetry of Spinal Cord White Matter Mitochondria via 3D Reconstruction from Conventional Transmission Electron Microscopy
Postmortem Human Brain Tissue Processing for Transmission Electron Microscopy of Gray Matter Myelin.
ISBN:
9781071654569

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