Pollen and pollen tube analysis : methods and protocols / edited by Giampiero Cai, Chiara Piccini, Veronica Conti, Sara Parri

Format:

Book

Contributor:

Cai, G. (Giampiero), 1963- editor.

Piccini, Chiara, editor.

Conti, Veronica, editor.

Parri, Sara, editor.

Series:

Methods in molecular biology ; v. 3042.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3042

Springer protocols

Language:

English

Subjects (All):

Palynology.

Pollen.

Pollen tube.

palynology.

pollen.

Physical Description:

1 online resource : illustrations (some color)

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume serves as an indispensable guide for researchers, students, and professionals who wish to explore the intricate world of pollen biology and its modern methodologies. Chapters detail collecting, counting, storing pollen, artificial intelligence, pollen classification, flow cytometric analysis, pollen characteristics, multispectral imaging, pollen tube growth in stiff media and measuring self-incompatibility in plants, providing solutions and methodologies for these obstacles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Pollen and Pollen Tube Analysis: Methods and Protocols represents a collaborative effort to advance the understanding of pollen biology using modern techniques and applications. This volume will serve as a valuable resource for those entering the fascinating field of pollen research"-- Springer Nature Link

Contents:

Assessment of pollen viability and germination through histochemical, in vivo and in vitro methods / Everton Hilo de Souza, Taliane Leila Soares, Mônica Lanzoni Rossi, Lidyanne Yuriko Saleme Aona, and Fernanda Vidigal Duarte Souza

Evaluating pollen viability and tube growth in rice (Oryza sativa) / Ji-Hyun Kim and Yu-Jin Kim

Analyzing pollen with multispectral imaging flow cytometry (MIFC) / Thomas Hornick, Franziska Walther, and Susanne Dunker

Imaging pollen and pollen tubes by scanning and transmission electron microscopy / Weiwei Zhang and Kefeng Fang

Visualizing pollen and pollen tube development using fluorescence microscopy / Ewa Szczuka and Agata Leszczuk

Automated measurements of fluorescence in spatially defined regions of pollen tubes / Jeremy Adler and Ingela Parmryd

Quantitative profiling of metabolites, sugars, and lipids in pollen tubes by mass spectrometry / Maneesh Lingwan, Neeta Lohani, and Erin Cullen

Analyzing pollen by infrared and Raman spectroscopy / Boris Zimmermann and Achim Kohler

Extracting and quantifying specific pollen proteins by Western blot / Sara Parri, Chiara Piccini, Giampiero Cai, and Veronica Conti

Detecting ROS production by pollen grains and tubes / Maria Breygina, Oksana Luneva, and Anna Podobedova

Quantitative phenotyping of ion fluxes in tip-growing cells under varying growth regimes : A data analysis protocol / Maria Teresa Portes, Daniel Santa Cruz Damineli, and José A. Feijó

Quantitatively analyzing the structural organization at the tip of growing tobacco pollen tubes / Carolin Fritz and Benedikt Kost

Implementing in vitro and in vivo approaches to analyze lipid–protein interactions in growing tobacco pollen tubes / Eliška Škrabálková, Ondřej Novotný, Přemysl Pejchar, and Martin Potocký

Evaluation of pectin and arabinogalactan protein distribution in olive pollen tube cell walls using immunofluorescent labeling / Aslıhan Çetinbaş-Genç

Evaluating self-incompatibility in citrus by histological and molecular assays / Stefania Bennici, Gaetano Distefano, and Alessandra Gentile

Comparing in vivo and in vitro pollen tube attraction assays for Arabidopsis and maize / Yifang Sui, Wantong Qu, Daniel Bouyer, Junyi Chen, and Thomas Dresselhaus

Employing biolistic transformation to investigate subcellular protein localization and interactions in growing tobacco pollen tubes / Patricia L. Seitz, Liang-Zi Zhou, and Thomas Dresselhaus

Isolation of proteins and extracellular vesicles secreted by pollen of Actinidia chinensis L. during in vitro germination / Chiara Suanno, Elisabetta A. M. Verderio, and Stefano Del Duca

Preparing a pollen reference collection and standardized protocols for aerobiological and melissopalynological pollen slides / Eleonora Clò, Paola Torri, Martina Denami, and Anna Maria Mercuri

Palynology : Extracting pollen from mosses and sediments / Jessica Zappa, Lorenzo Braga, Daniil Gornov, Eleonora Rattighieri, Cristina Ricucci, and Assunta Florenzano

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed August 31, 2026)

Other Format:

Print version: Pollen and pollen tube analysis

ISBN:

9781071653081

1071653083

OCLC:

1596674527

Publisher Number:

CIPO000393578

Access Restriction:

Restricted for use by site license