Gangliosides methods sand protocols edited by Koichi Furukawa

Format:

Book

Contributor:

Furukawa, Kōichi, Professor, editor.

Series:

Methods in molecular biology ; v. 3035.

Springer protocols (Series)

Methods in molecular biology 1940-6029 3035

Springer protocols

Language:

English

Subjects (All):

Gangliosides.

Medical Subjects:

Gangliosides.

Physical Description:

1 online resource

Place of Publication:

New York, NY Humana Press [2026]

Summary:

"This volume looks at the newest technology and approaches, along with classical methodologies, to thoroughly study Glycoscience and Ganglioside science. The chapters in this book cover topics such as synthesis and degradation of gangliosides including chemical synthesis, substrate preparation, and synthetic and degradation enzyme; ganglioside behaviors and biology including neutral glycolipids, single particle tracking, lipid raft analysis, and metabolic analysis in foods; and disease and tissue development including bone metabolism, melanocyte gangliosides, autoimmune neuropathies, and amyloid fibril formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Gangliosides: Methods and Protocols is a valuable resource for researchers and scientists who want to learn more about this important and developing field"-- Springer Nature Link

Contents:

Structure and structural properties of the gangliosides / Laura Mauri and Sandro Sonnino

Chemical synthesis of gangliosides: Synthesis of uniform ganglioside / Hiromune Ando and Akihiro Imamura

Chemical synthesis of fluorescent analogs of a- and b-series gangliosides / Naoko Komura and Hiromune Ando

Identification and evaluation of CMP-sialic acids / Di Wu, Takayuki Omoto, Chihiro Sato, and Ken Kitajima

Enzyme activity of glycolipid-specific glycosyltransferase enzymes GM2/GD2 synthase β1,4-GalNAc transferase (B4GALNT1) / Robiul Hasan Bhuiyan, Farhana Yesmin, Keiko Furukawa, Yuhsuke Ohmi, and Koichi Furukawa

Degradation system: Sialidase / Kazuhiro Shiozaki, Koji Yamamoto, and Taeko Miyagi

LC-MS-based analytical strategies for ganglioside profiling / Kazutaka Ikeda

Structure analysis of gangliosides: Mass spectrometry imaging / Ariful Islam and Mitsutoshi Setou

NMR of gangliosides / Yoshiki Yamaguchi

Analysis of ganglioside molecular species focusing on ceramide structures using thin-layer chromatography-mass spectrometry / Noboru Hashimoto, Shizuka Ito, and Akihito Yamamoto

Chemical identification of glycosphingolipid antigens recognized by antibodies / Koichi Furukawa, Yuhsuke Ohmi, Orie Tajima, and Keiko Furukawa

Elucidation of ganglioside microdomains using proximity labeling / Norihiro Kotani, Arisa Miyagawa-Yamaguchi, and Koichi Honke

Analysis of intracellular trafficking of cholera toxin B subunits depending on ganglioside GM1 in tissue-resident macrophages / Izumi Sasaki, Shiori Kaji, Naoko Wakaki-Nishiyama, and Tsuneyasu Kaisho

Electrostatic interaction analysis between GM3 and insulin receptor / Kazuya Kabayama

Methods for investigating Siglec-ganglioside interactions / Edward N. Schmidt and Matthew S. Macauley

Expression of mono- and dimeric ganglioside-binding lectins and their cellular functions / Kenichi Kamata, Marco Gerdol, Imtiaj Hasan, Sultana Rajia, S. M. Abe Kawsar, Tatsuya Kawasaki, Masao Yamada, Yukiko Ogawa, Yasuhiro Ozeki, Hideaki Fujita, and Yuki Fujii

Visualization of ganglioside-bacteria interaction using a frozen tissue overlay assay with GFP-labeled bacteria and ganglioside-specific antibodies / Shinichi Chisada, Kohei Shimizu, Naoyuki Matsunaga, Nozomu Okino, and Makoto Ito

Identification of gangliosides as bacterial attachment sites using a TLC-overlay assay with radioisotope-labeled bacteria / Shinichi Chisada, Naoyuki Matsunaga, Nozomu Okino, and Makoto Ito

Analyzing molecular complexes containing gangliosides via co-immunoprecipitation / Yuki Ohkawa

Analysis of lacto/neolacto-series glycolipids and gangliosides / Hidemi Arakawa and Akira Togayachi

Ganglioside functions in extracellular vesicles as revealed by single-particle tracking / Tatsuki Isogai, Miki Kanno, Koichi Furukawa, and Kenichi G. N. Suzuki

Small-scale cell biological analysis of lipid rafts of blood platelets and cerebellar neurons / Keisuke Komatsuya, Norihito Kikuchi, Tetsuya Hirabayashi, and Kohji Kasahara

Gangliosides and membrane microdomains / Motohide Murate, Noriko Yokoyama, Nario Tomishige, Nicolas Humbert, Ludovic Richert, Yves Mély, Kazuhisa Iwabuchi, and Toshihide Kobayashi

Metabolic analysis: Tracing gangliosides and precursors in foods / Tetsuya Okuda

Flow cytometry analysis of gangliosides expressed on extracellular vesicles / Yuhsuke Ohmi, Hiroka Koyanagi, Mohammad Abul Hasnat, and Keiko Furukawa

Methods for evaluation of the differentiation to osteoblasts and osteoclasts: Roles of gangliosides in bone metabolisms / Hisataka Kondo, Gyosuke Sakashita, Mayu Nagao, and Kazunori Hamamura

Ganglioside expression in melanocytes: Modulation by intrinsic and extrinsic factors / Keiko Furukawa and Orie Tajima

Gangliosides and autoimmune neuropathies / Motoi Kuwahara and Susumu Kusunoki

Imaging and characterization of ganglioside-induced amyloid fibril formation / Teruhiko Matsubara

Preparation of ganglioside-reconstituted liposomes / Asako Shimoda and Kazunari Akiyoshi

Intracerebroventricular administration of gangliosides for in vivo functional studies in mice / John Monyror, Melanie Horkey, Asifa K. Zaidi, and Simonetta Sipione

Gangliosides in neural stem cell fate determination and nerve cell specification: Preparation and administration / Yutaka Itokazu, Toshio Ariga, Takahiro Fuchigami, and Dongpei Li.

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed August 31, 2026)

Other Format:

Print version Gangliosides

ISBN:

9781071652886

1071652885

OCLC:

1612971143

Access Restriction:

Restricted for use by site license