Fusobacterium nucleatum : methods and protocols / edited by Hung Ton-That

Format:

Book

Contributor:

Ton-That, Hung, editor.

Series:

Methods in molecular biology ; v. 3055.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3055

Springer protocols

Language:

English

Subjects (All):

Fusobacterium.

Pathogenic bacteria.

Physical Description:

1 online resource : illustrations (some color)

illustration

polychrome

monochrome

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume presents a collection of both classic and modern experimental methods designed to highlight the biology, pathogenicity, and host interactions of Fusobacterium nucleatum. The chapters in this book provide practical guidance spanning microbiology, molecular biology, imaging, genetic engineering, metabolomics, and in vivo modeling. Some of the topics discussed in this volume are methods for visualizing and isolating F. nucleatum within its native oral environment; essential cellular processes such as surface protein assembly and peptidoglycan biosynthesis; protocols for asymmetric coculture systems; 3D tumor spheroid models; untargeted metabolite profiling of infected mammalian cells; and several in vivo platforms – from murine models of periodontitis, oral cancer, and CRC to a Caenorhabditis elegans infection model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Fusobacterium nucleatum: Methods and Protocols is a fundamental resource for both new and experienced researchers looking to investigate F. nucleatum virulence mechanisms, colonization strategies, and its increasing contributions to diseases such as CRC, oral squamous cell carcinoma, and periodontitis"-- Springer Nature Link

Contents:

CLASI-FISH imaging of Fusobacterium in oral microbial communities / Nicholas de Mojana di Cologna, Ruogu Wang, Alex A. Lemus, and Alex M. Valm

Isolation and characterization of Fusobacterium nucleatum subspecies from oral clinical specimens / Madeline Krieger, Christina Borland, and Justin Merritt

A droplet digital PCR approach for the quantitative detection of Fusobacterium nucleatum in formaldehyde-fixed, paraffin-embedded tumor tissues / Orsalem Kahsai, Amanda I. Phipps, Polly A. Newcomb, and Meredith A. J. Hullar

Enhanced transformation efficiency in Fusobacterium nucleatum by bypassing restriction-modification systems : A methylation-guided protocol / Ling Liu, Yu Zhang, and Xue Liu

A conditional plasmid system for markerless gene deletion in genetically recalcitrant Fusobacterium nucleatum subsp. animalis / Bibek G. C. and Chenggang Wu

Gene silencing by CRISPR interference in Fusobacterium nucleatum / Shiqi Xu, Bibek G. C., Kexin Tan, and Chenggang Wu

Methods for the evasion of restriction-modification systems during genetic engineering of Fusobacterium species / Martha A. Zepeda-Rivera, Elsa F. McMahon, Kaitlyn N. Lewis, Rutika Gavate, Falk Ponath, and Christopher D. Johnston

Tracking surface protein assembly and peptidoglycan biosynthesis in Fusobacterium nucleatum / Timmie A. Britton, Bryan Sanchez, Asis Das, and Hung Ton-That

Isolation of Fusobacterium nucleatum-induced host exosomal sRNAs / Ajay Kumar and Xuesong He

Isolation of primary RNA for differential RNA sequencing in Fusobacterium nucleatum / Falk Ponath and Jörg Vogel

Isolation and visualization of Fusobacterium nucleatum microcompartments / Dana Franklin, Angela Agnew, John Crow, Z. Hong Zhou, and Hung Ton-That

Isolation and proteomic characterization of outer membrane vesicles in Fusobacterium nucleatum / Jinjing Liu and Yung-Fu Chang

An asymmetric gas coculture system for oral pathobionts and gingival epithelial cells / Zeyang Pang, Collins I. Chimezie, Amber E. Haley, Nicole M. Cady, Lujia Cen, Thomas M. Schmidt, Xuesong He, and Jiahe Li

A 3D tumor spheroid platform to model Fusobacterium nucleatum-induced tumorigenesis / Yi-Wei Chen, Omri Kariv, and Hung Ton-That

Fluorescence and electron microscopy of Fusobacterium nucleatum-induced autophagy / Yimin Chen and Hung Ton-That

Mass spectrometry-based untargeted metabolite profiling to discover molecular consequences of mammalian cell infection with Fusobacterium nucleatum / Qiuying Chen, Huiyong Cheng, and Steven S. Gross

A mouse model for Fusobacterium nucleatum-mediated experimental periodontitis / Sharon Kim, Chungyu Chang, Hung Ton-That, No-Hee Park, and Reuben H. Kim

A murine floor-of-mouth model for investigation of Fusobacterium nucleatum-associated oral squamous cell carcinoma / Pachiyappan Kamarajan, Allan Radaic, and Yvonne L. Hernandez-Kapila

Modeling Fusobacterium nucleatum-driven colorectal cancer in Apc-mutant mice / Bing Han, Wei Liu, Yimin Chen, Yi-Wei Chen, Hung Ton-That, Jia Chang, and Cun-Yu Wang

Caenorhabditis elegans as a model host for Fusobacterium nucleatum / Suhani B. Bhakta and Yi-Wei Chen

Notes:

Includes bibliographical references

Online resource; title from PDF title page (Springer Nature Link, viewed August 31, 2026)

Other Format:

Print version: Fusobacterium nucleatum

ISBN:

9781071653883

1071653881

OCLC:

1596674332

Publisher Number:

CIPO000395761

Access Restriction:

Restricted for use by site license