Degrons methods and protocols edited by Maria W. Górna, Maria M. Klimecka, Matylda A. Izert-Nowakowska

Format:

Book

Contributor:

Górna, Maria W. (Maria Wiktoria), editor.

Klimecka, Maria M., editor.

Izert-Nowakowska, Matylda A., editor.

Series:

Methods in molecular biology ; v. 3069.

Springer protocols (Series)

Methods in molecular biology 1940-6029 3069

Springer protocols

Language:

English

Subjects (All):

Amino acid sequence.

Amino Acid Sequence.

Medical Subjects:

Amino Acid Sequence.

Physical Description:

1 online resource

Place of Publication:

New York, NY Humana Press [2026]

Summary:

"This detailed volume provides protocols for the identification, characterization, and application of degrons, central to controlling protein abundance and cellular homeostasis, in a range of organisms from bacteria to humans. Beginning with reporter assays and tools used in human cells to study the involvement of the ubiquitin-proteasome system or ubiquitin-independent degradation pathways, the book continues by exploring methods for chemical and enzymatic synthesis, characterization of degrons and their mimics, methods for other selected eukaryotes, including plants and employing inducible degradation in nematodes and trypanosomes, as well as methods used for studying N- and C-degrons in bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls"-- Springer Nature Link

Contents:

HiBiT-RR and nLucK0 tagging systems for monitoring targeted protein degradation / Hanfeng Lin, Xiaoli Qi and Jin Wang

Identification of a ubiquitin-independent degron by a reporter assay / Yosef Shaul

Utilization of functional degradation pathways for the characterization of ubiquitin-proteasome system tool compounds / Julia M. Frischkorn, Kristin Riching, Stefan Knapp and Martin P. Schwalm

UbiSite approach : global site-specific profiling of canonical and non-canonical ubiquitination in cells and tissues / Nicoline Kingo Pedersen, Vyacheslav Akimov and Blagoy Blagoev

Chemical and enzymatic approaches to C-terminal cyclic imide formation / Zhenguang Zhao, Ethan Yang Feng, Wenqing Xu and Christina M. Woo

Design and semisynthesis of ubiquitin extension probes / Chuntong Li, Luyu Shi, Yingyue Zhang and Jinghong Li

Biophysical and cellular assays for the evaluation of peptidomimetic inhibitors based on degron sequences / Christopher Lenz, Krishna Saxena and Stefan Knapp

Multicolor live-cell mRNA imaging using RNA-regulated destabilization domains / Tien G. Pham, Omoyemi Ajayi and Jiahui Wu

Dynamic control of cell signaling with optogenetic modulation of protein abundance in living mammalian cells / Yu-En Huang, Payel Mondal, Huaxun Fan and Kai Zhang

Reporter gene assay for monitoring BCL6 reactivation / Catherine L. Ingram, Radosław P. Nowak and Thomas M. Geiger

Studying glycan-dependent ERAD of misfolded glycoproteins in plants / Ulrike Vavra, Christiane Veit and Richard Strasser

Protein depletion in Caenorhabditis elegans using the auxin-inducible degradation system / Jordan D. Ward

An auxin-inducible degron system for trypanosomes / Alexandra Klein, Alice McDowell, Christian R. S. Reis, Rodrigo Pontes de Lima, Danielle M. N. Moura, Janaina de Freitas Nascimento, Osvaldo P. de Melo Neto and Mark Carrington

Degrade green fluorescent protein (deGradFP) method in Trypanosoma brucei / Midori Ishii and Bungo Akiyoshi

Production of purified proteins tagged with ssrA-derived degrons and their degradation by the Escherichia coli ClpXP system / Maria M. Klimecka, Matylda A. Izert-Nowakowska and Maria W. Górna

Evaluation of degron motifs in Escherichia coli using a fluorescent reporter / Matylda A. Izert-Nowakowska, Patrycja E. Szybowska, Maria M. Klimecka and Maria W. Górna

Assessment of bacterial N-degron proteolysis by a dual-fluorescence reporter / Pratistha Kandel, Patrick C. Beardslee and Karl R. Schmitz

SsrA-based targeted protein degradation in Escherichia coli / Qichang Nie and Terrence Chi-Kong Lau

Notes:

Includes bibliographical references

Online resource; title from PDF title page (Springer Nature Link, viewed August 14, 2026)

Other Format:

Print version Degrons

ISBN:

9781071655085

1071655086

OCLC:

1611145741

Access Restriction:

Restricted for use by site license