Targeting enzymes for pharmaceutical development : methods and protocols

Format:

Book

Contributor:

Labrou, Nikolaos E., editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 3021.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3021

Springer protocols

Language:

English

Subjects (All):

Drug development.

Enzymes--Therapeutic use.

Enzymes.

Enzymology.

Physical Description:

1 online resource : illustrations

black and white

illustrations

Edition:

Second edition / edited by Nikolaos Labrou

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This second edition volume expands on the previous edition with a discussion of the latest experimental and computational strategies used in the study, characterization, and targeting of enzymes with therapeutic potential. The chapters in the book are organized into two parts: Part One presents detailed, reproducible protocols for enzyme characterization, inhibitor screening, and biochemical analysis. Part Two is dedicated to computational and in silico methodologies, reflecting the growing impact of computational biology in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Targeting Enzymes for Pharmaceutical Development: Methods and Protocols, Second Edition is a valuable resource for academic and industrial scientists in medicinal chemistry, chemical biology, pharmaceutical biotechnology, structural biology, and computational drug discovery. This book is both a technical manual and conceptual guide for scientists engaged in enzyme-related drug discovery and development"-- Springer Nature Link

Contents:

Measuring protein–ligand binding by thermal shift assay / Mohammad Khan and Anna Roujenkova

Applications of differential scanning fluorimetry and related technologies in characterization of protein–ligand interactions / Bolormaa Baljinnyam, Michael Ronzett, Adam Yasgar, and Anton Simeonov

Enzyme–ligand interaction monitored by synchrotron radiation circular dichroism / Rohanah Hussan, Charlotte S. Hughes, and Giuliano Siligardi

Binding interaction analysis of lysozyme and brilliant blue using surface plasmon resonance / Zeynep Aleyna Kahraman, Sule Coskun, Simay Ungur, Ilgaz Tastekin, and Emrullah Alper Türkoglu

Evaluation of endolysin activity using nephelometric assays with whole bacterial cells and isolated peptidoglycan / Panagiota D. Pantiora, Nikolaos D. Georgakis, Georgios E. Premets, Christina Variotsou, Eleni Pavlou, Alexandra Sklavou, Irene Axarl, and Nikolaos E. Labrou

In vitro assays for endolysin efficacy and safety profiling / Nikolaos D. Georgakis, Panagiota D. Pantiora, Georgios E. Premets, Christina Variotsou, Eleni Pavlou, Alexandra Sklavou, Irene Axarl, and Nikolaos E. Labrou

Droplet-based microfluidics methods for detecting enzyme inhibitors / Abraham Ochoa, Frida Trejo, and Luis F. Olguín

HMG-CoA reductase inhibition assays : Updated protocols for drug discovery and enzyme kinetics / Baskaran Gunasekaran, Shamala Selvaman, Valdéh Ulaganathan, Saema Hanafi, and Mohd Yunus Shukor

The enzyme-bag platform for cytochrome P450 biotransformation and high-throughput drug metabolism screening / Pradeepraj Durairaj

Enzymatic bioautographic methods / I. Ayelen Ramallo, Mario O. Salazar, and Ricardo L. E. Furlan

On-line enantioselective capillary electrophoretic method for screening cytochrome P450 3A4 inhibitors / Roman Remínek, Zdenek Glatz, and Wolfgang Thormann

Isolation and endopeptidase inhibition of human a2-macroglobulin / Soraia R. Mendes, F. Xavier Gomis-Rüth, and Theodoros Goulas

Harnessing affinity tags for recombinant protein purification : Concepts, methods, and troubleshooting / Oshadi Edrisinghe, Kainat Shahzad, Lawrenca Kum, Zena Alraawi, Gaétane Terrier, Patience Salvalina Okoto, Paul Adams, and Thallapuranam Krishnaswamy Suresh Kumar

Computational strategies for discovering inhibitors targeting therapeutic enzymes / Anand Kumar Pandey

Computational study of enzyme inhibition / Francisco das Chagas Pereira Andrade, Mateus Henrique de Almeida Costa, and Anderson Nogueira Mendes

In silico laboratory : New and updated tools for protein-centered drug discovery / Dušanka Janežic and Janez Konc

Combined in silico approaches for virtual screening of noncovalent immunoproteasome inhibitors for multiple myeloma treatment / Giulia Culletta, Muhammad Sohaib Roomi, Lisa Longo, and Marco Tutone

Deep learning pipeline for accelerating virtual screening in drug discovery / Fatma Noor and Muhammad Tahir Ul Qamar

In silico methodology for human carboxylesterases 1 and 2 isoforms substrate selectivity study / Sergio R. Ribone

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed August 19, 2026)

Other Format:

Print version: Targeting enzymes for pharmaceutical development

ISBN:

9781071651902

1071651900

OCLC:

1611899341

Publisher Number:

CIPO000350277

Access Restriction:

Restricted for use by site license