Stem cell-based human embryo models : methods and protocols / edited by Vincent Pasque, Kursad Turksen

Format:

Book

Contributor:

Pasque, Vincent, editor.

Turksen, Kursad, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 3048.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3048

Springer protocols

Language:

English

Subjects (All):

Embryonic stem cells--Laboratory manuals.

Embryonic stem cells.

Human embryo--Laboratory manuals.

Human embryo.

Embryo, Mammalian.

Medical Subjects:

Embryo, Mammalian.

Physical Description:

1 online resource : illustrations

black and white

illustrations

Place of Publication:

New York, NY : Huaman Press, [2026]

Summary:

"This detailed volume covers diverse aspects of stem cell research and exemplifies some of the developments and improvements in the field. The book explores how to regulate and manipulate stem cells appropriately in vitro, allowing for the generation of very early embryo-like structures, known as blastoids, opening up opportunities for analyzing and delineating the very early steps in embryo development under controlled in vitro conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell-Based Human Embryo Models: Methods and Protocols serves as an ideal guide for researchers looking to drive rapid innovations in the study of early embryonic development"-- Springer Nature Link

Contents:

Human stem cell-based embryo model research : Ethics and regulations / Chie Kobayashi, Shu Ishida, and Tsutomu Sawai

From single stem cells to an in vitro model of the post-implantation human embryo : A step-by-step guide / Seher Ipek Gassaloglu, Monique Pedroza, and Berna Sozen

Generating human blastoids following transient naïve resetting of primed pluripotent stem cells / Maria Carolina Zimara, Carlos A. Pinzon-Arteaga, Kun Liu, Toshihiko Ezashi, Seiya Oura, Shijian Lyu, Menaka Sanghvi, Ye Yuan, and Jun Wu

Generation of blastoids from human naïve pluripotent stem cells / Hiroo Sasaki, Iori Sasaki, Jennifer Nichols, and Ayaka Yanagid

Use of miR-200-mediated reprogramming and mechanical cues to generate human blastocyst models / Sharon Arcuri, Georgia Pennarossa, Fulvio Gandolfi, and Tiziana A. L. Brevini

Generation of the nonintegrated human bilaminar embryo model (Bilaminoid) from naïve pluripotent stem cells / Takumi Okubo and Yasuhiro Takashima

Modeling somitogenesis and axial development in vitro with axioloids / Keun-Tae Kim, Sofiane Hamidi, and Cantas Alev

From stem cells to post-gastrulation amnioids (PGAs) : A protocol to model human extra-embryonic development / Borzo Gharibi and Silvia D. M. Santos

Interaction between pluripotent stem cells and amniotic epithelium for the in vitro study of human embryogenesis / Daniela Ávila-González, Axel Castro-Abrego, Jonathan Salazar-Alonso, Omar Martínez Alarcon, Alejandro Martinez-Juarez, Anayansi Molina Hernández, Nestor Emmanuel Diaz-Martinez, Wendy Portillo, and Nestor Fabián Diaz

Extended in vitro culture of human embryos / Sylwia M. Czukiewska, Felicitas Azpiroz, Susana M. Chuva de Sousa Lopes, and Mina Popovic

Generation of human naïve pluripotent stem cell-derived blastoids in thermoformed microwell platforms / Dorian G. Luijkx, Erik J. Vrij, Ge Guo, and Stefan Giselbrecht

Microfluidic on-chip assay for quantifying blastoid adhesion to endometrial epithelium / Asli Ak, Dorian Luijkx, Daniel Carvalho, Stefan Giselbrecht, Ron van Golde, and Erik Vrij

Linking single-cell dynamics to cell fate in differentiating hPSCs / Seth Teague, Zhiyuan Yu, and Idse Heemskerk

Application of CRISPR-based epigenome editing tools for engineering programmable embryo models / Gerrald A. Lodewijk, Sayaka Kozuki, Carly Guiltinan, Benjamin R. Topacio, and S. Ali Shariati

Direct reprogramming of human fibroblasts into fully functional trophoblast stem cells / Meir Azagury and Yosef Buganim

3D in vitro culture of early mouse embryos and trophoblast tissue explants / Devila Prit, Shashank Jaitly, Niraimathi Govindasamy, Adrian Ranga, and Ivan Bedzhov

Generation of porcine blastoids from embryonic stem cells / Jinzhu Xiang, Hanning Wang, and Duanqing Pei

A deep learning approach to assessing cell identity in stem cell-based embryo models / Nazmus Salehin, Martin Proks, and Joshua M. Brickman

An optimized protocol for the extended culture of gastruloids from mouse embryonic stem cells / Marloes Blotenburg, Shruthi Buddharaju, Beril Esin, and Peter Zeller

Optimization of gastruloid pre-culture conditions for reproducible germ layer composition / Marloes Blotenburg, Beril Esin, Shruthi Buddharaju, and Peter Zeller

Microfluidic device manufacturing by light-based 3D printing for organoid vascularization / Rochelle Aubry, Idris Salmon, and Adrian Ranga

Engineering synthetic PEG hydrogels to model peri-implantation epiblast morphogenesis with tunable biophysical properties / Michael Patrick Seitz, Zhen Ma, and Era Jain

Notes:

Includes bibliographical references

Online resource; title from PDF title page (Springer Nature Link, viewed August 19, 2026)

Other Format:

Print version: Stem cell-based human embryo models

ISBN:

9781071653562

1071653563

OCLC:

1611900892

Publisher Number:

CIPO000409496

Access Restriction:

Restricted for use by site license