Bioconjugation methods / edited by Alexander Deiters

Format:

Book

Contributor:

Deiters, Alexander, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 3009.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3009

Springer protocols

Language:

English

Subjects (All):

Bioconjugates.

Physical Description:

1 online resource : illustrations (black and white, and colour)

black and white

color

illustrations

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume presents cutting-edge chemical and molecular strategies. Chapters detail bioorthogonal cycloadditions, electrophiles, self‑labeling systems, noncanonical amino acids, site‑selective bioconjugation across proteins, nucleic acids, cells, and viral assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Bioconjugation Methods is a valuable resource for all researchers who are looking to learn more about this important and advancing field"-- Springer Nature Link

Contents:

Site-specific chemical conjugation of recombinant proteins onto the AAV capsid / Quan Pham, Jake Glicksman and Abhishek Chatterjee

Bioorthogonal surface modification of measles and vaccinia viruses using metabolic azide reporters / Shan Hu, Thongpon Meethong, Monchupa Kingsak and Qian Wang

Synthesis of linkage-specific polyubiquitin probes using unnatural amino acids / Matthew T. Buhl and T. Ashton Cropp

Synthesis of Ub-ACA, a fluorogenic DUB substrate via ACPL method / Saibal Chanda and Wenshe Ray Liu

Native chemical ligation to install chemical protein modifications / Eldon R. Hard, Afraah Javed and Matthew R. Pratt

Efficient and site-specific protein bioconjugation quantification in mammalian cells / Gabriella Belsito, Olivia Shade and Alexander Deiters

Intelligence disclosure on deploying SpyTag/SpyCatcher for nanoparticle assembly / Rory A. Hills and Mark R. Howarth

Rapid and wash-free live cell labeling with BenzoTag / Bryan J. Lampkin

Antibody conjugation to cell surface receptors / Avani Parikh, Elisa Ruffo, Alexander Deiters and Jason Lohmueller

Cysteine re-bridging using divinyl pyrimidine reagents for the generation of antibody-drug conjugates / Thomas Wharton, Sona Krajcovicova and David R. Spring

Sortase A catalyzed transpeptidation for protein bioengineering / Floris J. van Dalen and Hidde L. Ploegh

Attachment of 2-pyridyltetrazine affinity bioorthogonal chemistry (ABC) tags to proteins for site-selective bioorthogonal chemistry / Paramesh K. Ramaraj, Samuel L. Scinto and Joseph M. Fox

N-acyl-N-alkyl sulfonamide mediated bioconjugation of His-tag fused proteins / Vikram Thimaradka, Tomonori Tamura and Itaru Hamachi

Chemoselective histidine bioconjugation using thiophosphorodichloridate reagents / Xinyuan Li, Fung Kit Tang and Shang Jia

Reactive DNA probes for cross-linking with specific amino acids of protein / Denise-Liu’ Gracias Leone, Ambra Spampinato and Michal Hocek

Protein modification via bioorthogonal HS-BCN ligation / Ming Fang and Qing Lin

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed August 11, 2026)

Other Format:

Print version: Bioconjugation methods

ISBN:

9781071651124

1071651129

OCLC:

1592132224

Publisher Number:

CIPO000382307

Access Restriction:

Restricted for use by site license