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The innate cytokine il-18 inhibits cns autoimmunity through preferential activation of protective cd8 t-cells Jeremy A Morrissette

Dissertations & Theses @ University of Pennsylvania Available online

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Format:
Book
Thesis/Dissertation
Author/Creator:
Morrissette, Jeremy A., author.
Contributor:
University of Pennsylvania. Immunology., degree granting institution.
Language:
English
Subjects (All):
Immunology.
Cellular biology.
Pathology.
0982.
0379.
0571.
Local Subjects:
Immunology.
Cellular biology.
Pathology.
0982.
0379.
0571.
Genre:
Academic theses
Physical Description:
1 online resource (106 pages)
Contained In:
Dissertations Abstracts International 87-12B
Place of Publication:
Ann Arbor : ProQuest Dissertations and Theses, 2026
Language Note:
English
Summary:
Chronic innate immune activation is widely thought to challenge self-tolerance. IL-18 is an innate, inflammasome-activated cytokine and potent amplifier of T-cell activation. In excess, IL-18 is associated with certain autoinflammatory, but not autoimmune, diseases. We tested how excess IL-18 affected susceptibility to experimental autoimmune encephalomyelitis (EAE), a model of CNS autoimmunity driven by IL-18 responsive, myelin-autoreactive CD4T-cells (CD4Tauto). Prior data suggested that IL-18 would exacerbate immunopathology in EAE. Instead, excess IL-18 was profoundly protective. Excess IL-18 did not impair CD4Tauto priming or early expansion. Rather, it selectively restricted later accumulation of highly activated, splenic CD4Tauto bearing CNS-homing integrins. Despite high IL-18 receptor expression on CD4Tauto and FOXP3+ CD4Treg, excess IL-18 acted specifically through mature CD8T-cells to promote a highly activated CD8Teffector phenotype and IFNγ-dependent protection from EAE. Therapeutic administration of a "decoy-resistant" IL-18 agonist (DR-18) to wild-type mice, even after CD4Tauto expansion, engaged CD8T-cells to diminish CD4Tauto abundance, prevent CNS infiltration, and block immunopathology. Together, these findings demonstrate the unexpected, dominant ability of IL-18 to mobilize protective CD8T-cells against highly activated CD4Tauto and protect from CNS autoimmune pathology; illustrating a potential therapeutically relevant mechanism by which autoinflammation actively opposes autoimmunity
Notes:
Source: Dissertations Abstracts International, Volume: 87-12, Section: B.
Advisors: Canna, Scott W. Committee members: Jordan, Martha S.; Hunter, Christopher A.; Bar-Or, Amit; Anguera, Montserrat C.
Ph.D. University of Pennsylvania 2026
Vendor supplied data
Local Notes:
School code: 0175
ISBN:
9798247983095
Access Restriction:
Restricted for use by site license

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