1 option
Development of a modular platform for intracellular post-translational modifications editing Brianna Hill-Payne
- Format:
- Book
- Thesis/Dissertation
- Author/Creator:
- Hill-Payne, Brianna, author.
- Language:
- English
- Subjects (All):
- Biochemistry.
- Molecular biology.
- Biophysics.
- 0487.
- 0307.
- 0786.
- Local Subjects:
- Biochemistry.
- Molecular biology.
- Biophysics.
- 0487.
- 0307.
- 0786.
- Genre:
- Academic theses
- Physical Description:
- 1 online resource (170 pages)
- Contained In:
- Dissertations Abstracts International 87-12B
- Place of Publication:
- Ann Arbor : ProQuest Dissertations and Theses, 2026
- Language Note:
- English
- Summary:
- The regulation of post-translational modifications (PTMs) is central to cellular biology and disease. Induced-proximity strategies enable manipulation of PTMs by recruiting modifying enzymes to proteins of interest, but identifying effective effector enzymes typically requires extensive heterobifunctional molecule synthesis before biological validation. Here we report a modular platform that enables rapid evaluation of PTM editing enzymes against defined protein substrates in living cells using compound-dependent or nanobody-mediated induced proximity. Using lysine acetylation as a model system, we demonstrate programmable acetylation of GFP, histone H3, and p53 through recruitment of diverse acetyltransferases. Effector identity dictates site-specific acetylation patterns, enabling selective PTM deposition across substrates and cellular compartments. This platform enables rapid identification of productive effector-substrate relationships prior to heterobifunctional molecule development, accelerating the design of induced-proximity chemical probes for targeted PTM editing
- Notes:
- Source: Dissertations Abstracts International, Volume: 87-12, Section: B.
- Advisors: Burslem, George M. Committee members: Marmorstein, Ronen; Korb, Erica; Sellmyer, Mark; Paralkar, Vikram; Phillips, Richard
- Ph.D. University of Pennsylvania 2026
- Vendor supplied data
- Local Notes:
- School code: 0175
- ISBN:
- 9798247979531
- Access Restriction:
- Restricted for use by site license
The Penn Libraries is committed to describing library materials using current, accurate, and responsible language. If you discover outdated or inaccurate language, please fill out this feedback form to report it and suggest alternative language.