Synthetic gene circuits : methods and protocols / edited by Xiao-Jun Tian

Format:

Book

Contributor:

Tian, Xiao-Jun, editor.

Series:

Methods in molecular biology ; v. 3041.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3041

Springer protocols

Language:

English

Subjects (All):

Gene regulatory networks.

Physical Description:

1 online resource : illustrations (some color)

black and white

color

illustrations

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This detailed volume presents experimental and computational methods for constructing and interrogating synthetic gene circuits across multiple organizational scales. Beginning with a section on genetic tools, platforms, and experimental frameworks that enable sophisticated circuit construction, the book continues by covering methods for regulating circuit behavior and mitigating variability, as well as frameworks for understanding time-dependent circuit behavior in growing cells and populations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Gene Circuits: Methods and Protocols aims to support a synthetic biology practice that is predictive by design, robust by control, and interpretable through dynamics"-- Springer Nature Link

Contents:

Reimagining the synthetic biology dbtl cycle with machine learning / Matthew Lee, Yiduo Wang, Kshitij Rai, Shuo Li, Hannah K. Whited, Mateo A. Pozo Araujo, and Caleb J. Bashor

A golden gate-compatible crispr-associated transposon tool for multiplexed bacterial genome editing / Thea C. T. Irvine, Andrew M. Bailey, and Thomas E. Gorochowski

Construction of a tl-crispri genetic circuit in bacteria for translation-level gene knockdown / Giho Kim, Ho Joon Kim, and Sang Woo Seo

Design of crispri-based synthetic gene circuits in bacteria / Javier Santos-Moreno

Design of conditional guide rnas for the logical regulation of gene expression / Dongwon Park, Woosub Shin, Hansol Kang, and Jongmin Kim

Synthetic biomolecular condensates : design principles and applications / Yuefeng Ma, Leshan Yang, and Yifan Dai

Characterizing optogenetic tools for use in synthetic gene circuits / Timothy H. Wakiyama and Megan N. McClean

Characterization of synthetic gene circuits with absolute quantification in continuous culture / Scott B. Stacey, Ting An Lee, Olivia Gallup, Eszter Csibra, Antonis Papachristodoulou, Harrison Steel, and Kirill Sechkar

Cell-free genetic circuits : extract and template preparation / Thales R. Spartalis, Kassidy B. Porche, Xun Tang, and Yong-Chan Kwon

Programming rna trans-splicing for versatile gene regulation and complex cellular logic computation / Yuanli Gao and Baojun Wang

Intercellular crispri for distributed genetic circuits / Abhinav Pujar, Anchita Sharma, and Manish Kushwaha

Feedback control and sensitivity analysis in synthetic gene circuits / Ania-Ariadna Baetica

Dynamic robust control of microbial communities using cybergenetics / Ting An Lee, Scott B. Stacey, Olivia Gallup, Harrison Steel, and Kirill Sechkar

From negative feedback to integrated control : recombinase-based strategies for mitigating resource competition / Rixin Zhang, Rong Zhang, and Xiao-Jun Tian

Harnessing crispri competition to develop multimodule controllers for resource-aware circuit design / Sadikshya Rijal, Rong Zhang, and Xiao-Jun Tian

Modeling growth-mediated dilution in synthetic circuits : effects of circuit topology and phase separation / Xiao-Jun Tian and Rong Zhang

Quantitative coarse-grained modeling of microbial metabolism : uncovering growth laws, environmental responses, and host-circuit coupling / Yi-Hui Lai and Ting Lu

Gene expression across growth stages (geags) : modeling circuit dynamics in batch cultures / Chelsea Y. Hu and Hari R. Namboothiri

Coupled perturbations of gene circuit dynamics by resource competition and growth dilution / Abdelrahaman Youssef, Rong Zhang, and Xiao-Jun Tian

Dynamic constrained allocation flux balance analysis (dcafba) / Huili Yuan, Yang Bai, and Xiongfei Fu

Constructing an nf-kb oscillator for dual-input control in yeast / Yuanxu Jiang, Yingying Fan, Zhibo Zhang, and Ping Wei

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed July 16, 2026)

Other Format:

Print version: Synthetic gene circuits

ISBN:

9781071653043

1071653040

OCLC:

1596674444

Publisher Number:

CIPO000393576

Access Restriction:

Restricted for use by site license