Circulating tumor cells : methods and protocols / edited by Mark Jesus Magbanua

Format:

Book

Contributor:

Magbanua, Mark Jesus M., editor.

Series:

Methods in molecular biology ; v. 2999.

Methods in molecular biology, 1940-6029 ; 2999

Language:

English

Subjects (All):

Tumor markers.

Cancer cells.

Neoplastic Cells, Circulating.

Biomarkers, Tumor--analysis.

Cell Separation--methods.

Biomarkers, Tumor.

Medical Subjects:

Neoplastic Cells, Circulating.

Biomarkers, Tumor--analysis.

Cell Separation--methods.

Biomarkers, Tumor.

Genre:

Laboratory manuals

Laboratory manuals.

Physical Description:

1 online resource : illustrations (black and white)

Edition:

Second edition

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This second editions explores new and updated approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Chapters detail comprehensive protocols for the capture of CTCs via filtration, density gradient centrifugation, microfluidic, immunomagnetic separation of CTCs, detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, flow cytometry, assays designed for genomic characterization, and functional analyses of CTCs. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls"-- Springer Nature Link

Contents:

Detection and separation of circulating tumor cells using magnetic fluorescent nanoparticles / Shuiling Chen, Yuwei Zhou, Shaobing Zhou, Xia Liu and Jianwen Hou

Optimized workflow for sensitive detection of circulating tumor cells using qPCR and pre-amplification to minimize cross-contamination / Eva Obermayr and Robert Zeillinger

Highly efficient capture of sarcoma cells in microfluidic devices / Yang Zhang, Joanne P. Lagmay and Z. Hugh Fan

Detection of circulating tumor cells with an epithelial-to-mesenchymal transition phenotype / Dominika Šmicková Rusnáková, Miroslav Tomáš, Daniel Pindák and Michal Mego

Phenotypic characterization and sorting of circulating tumor cells (CTCs) using a digital image-based sorter / Tania Rossi, Paola Ulivi and Michele Zanoni

A new filter for isolation of circulating tumor cells by only blood filtration / Noriyoshi Sawabata, Kohei Morita, Takeshi Nishikawa, Shigenobu Tatsumi, Hisae Suzuki, Toru Arakane, Taro Kobayashi, Maiko Takeda and Akihiko Yoshizawa

Androgen receptor variant 7 (AR-V7) transcript expression in circulating tumor cells / Tanzila Khan, Yafeng Ma, Alison Luk, Francis Young, Paul de Souza, Wei Chua, Bavanthi Balakrishnar and Therese M. Becker

Androgen receptor variant 7 (AR-V7) protein detection in circulating tumor cells by immunocytostaining / Tanzila Khan, Yafeng Ma, John Lock, Paul de Souza, David Harman, Wei Chua, Bavanthi Balakrishnar, Kieran Scott and Therese M. Becker

Androgen receptor splice variant-7 expression analysis in circulating tumor cells : methodology and application of AdnaTest in prostate cancer research / Naoya Nagaya and Shigeo Horie

Nanomechanical phenotyping of circulating tumor cells with atomic force microscopy / Pawel A. Osmulski, Yusheng Qian and Maria Gaczynska

Model circulating tumor cells induced by mechanical stress / Yusheng Qian, Pawel A. Osmulski and Maria Gaczynska

Characterization of circulating tumor cells and other rare cells in blood using the RareCyte platform / Arturo B. Ramirez, Jon Ladd, Erin G. Bayer, Brock Bartels, Casey Helmicki, Rachel N. Ponting, Daniel E. Campton, Joshua J. Nordberg and Eric P. Kaldjian

Liquid biopsies and molecular imaging for precise cancer management / Angelo Castello, Matteo Caracciolo, Mirco Bartolomei, Massimo Castellani and Egesta Lopci

Small cell lung cancer : screening of kinase inhibitors / Hamilton Gerhard, Marie-Therese Eggerstorfer and Sandra Stickler

Cell-interaction analysis and functional studies using circulating tumor cell models / Hubing Shi and Jinen Song

Negative enrichment and isolation of circulating tumor cells from cryopreserved blood fractions for whole genome amplification / Melanie Dawe and Susan J. Done

Isolation and sequencing of single circulating tumor cells / Marianne Oulhen, Maria Virginia Sanchez-Becerra, Patrycja Pawlikowska and Françoise Farace

Micro RNA sequencing in circulating tumor cells / Sarah M. Hücker and Stefan Kirsch

Single-cell RNA sequencing of pancreatic circulating tumor cells / Lei Zhu and Sebastian Schölch

Utilizing magnetic microraft technology for single-cell isolation of CTCs from whole blood for scRNA-Seq transcriptomic characterization / Terri Clister, Rosalyn M. Fey, Zachary R. Garrison, Khanh Doan, Heather Branchaud, Connor M. Hall, Rebecca A. Nichols and Rajan P. Kulkarni

Advancements in circulating tumor cell expansion ex vivo : a matured technology? / Jerry Xiao and Seema Agarwal

Culture and expansion of circulating tumor cells from breast malignant effusions as organoids / Amirabbas Parizadeh, Shruti Warhadpande, Mark Jesus M. Magbanua and Jennifer M. Rosenbluth

Bioinformatics of shallow whole genome sequencing data of circulating tumor cells to inform cancer diagnosis and treatment / Marco Silvestri, Carolina Reduzzi, Cinzia De Marco, Massimo Cristofanilli, Giancarlo Pruneri and Serena Di Cosimo

Automated analysis of liquid biopsy using deep learning : detecting circulating tumor cells and cancer-associated fibroblasts / Cheng Shen, Haowen Zhou, Siyu Lin, Anthony Williams, Changhuei Yang and Richard J. Cote

Artificial intelligence for CELLSEARCH image analysis / Frank A.W. Coumans, Nikolas H. Stoecklein and Leon W.M.M. Terstappen

RNA-Seq and XAI can be used as tools to aid pathologists in the process of cancer diagnosis / Patricia Porras-Quesada, Pilar Sánchez, Carmen M. Morales-Álvarez, Alberto Ramírez-Mena, María Jesús Álvarez-Cubero and Luis Javier Martínez-González

New strategies to uncover genes involved in the development of prostate cancer in families with multiple cases / Lucía Chica-Redecillas, Beatriz Álvarez-González, Sergio Cuenca-López, Maria Jesus Alvarez-Cubero and Luis Javier Martínez-González

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed July 13, 2026)

Other Format:

Print version: Circulating tumor cells

ISBN:

9781071650509

1071650505

OCLC:

1579001174

Publisher Number:

CIPO000346634

Access Restriction:

Restricted for use by site license