Chimeric RNAs : methods and protocols / edited by Sarah Lynch, Hui Li

Format:

Book

Contributor:

Lynch, Sarah Anne, 1994- editor.

Li, Hui (Associate professor of pathology), editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 3024.

Methods in molecular biology, 1940-6029 ; 3024

Language:

English

Subjects (All):

RNA.

Chimerism.

Medical Subjects:

RNA.

Chimerism.

Physical Description:

1 online resource : illustrations.

Edition:

Second edition.

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This detailed volume presents a comprehensive methodological guide for the study of chimeric RNAs, from their discovery in high-throughput datasets to their validation in the lab and characterization in biological and clinical contexts. Beginning with chapters dedicated to the identification and characterization of chimeric RNAs using numerous bioinformatic tools, this book continues by covering experimental validation and quantification of chimeric RNAs, methods for chimeric RNA functional and mechanistic studies, and translational application of chimeric RNAs and case studies. Written for the highly successful Methods in Molecular Biology series, chapters generally include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chimeric RNAs: Methods and Protocols, Second Edition serves as an ideal guide to the practical guidance and conceptual grounding needed to advance your work in this increasingly vital area of study"-- Springer Nature Link

Contents:

Integrative chimeric RNA prediction with FASfuse / Samir Lalani, Marina Wang and Hui Li

Identification of cross-strand chimeric RNAs with cscMap / Yuting Wang and Xuerui Yang

Detection of chimeric RNAs from RNA-Seq data with ChiTaRS 8.0 : Insights for liquid biopsy and drug target identification / Dylan D’Souza, Daniel Sumbatian, Bar Sever, Itamar Altman, Yonit Leykind and Milana Frenkel-Morgenstern

Computational prediction of chimeric RNAs from long reads using CTAT-LR-Fusion / Shafaque Zahra and Hui Li

Assembly of a full-length chimeric RNA transcriptome / Justin Elfman and Hui Li

AtFusionDB : A comprehensive database of fusion transcripts in model plant Arabidopsis thaliana / Tanu Shree and Shailesh Kumar

Detection and measurement of chimeric RNAs by RT-PCR using novel RNA extraction method / Anam Tajammal, Samuel Haddox and Hui Li

Mass spectrometry-based validation of chimeric RNAs / Shafaque Zahra, Justin Elfman and Hui Li

Validation of plant fusion peptides using proteomics data / Fiza Hamid, Sahrish Aftab, Tanu Shree and Shailesh Kumar

Calculating relative chimeric RNA expression with FusionBlaster / Samuel Haddox, Yuhang Mao and Hui Li

Ribosome RNA extraction and RNase R treatment / Qiong Wang, Yiwei Wang and Hui Li

Overexpression of chimeric RNA by retroviral transduction / Liang Cheng, Chenwei Yang, XiaoLe Lu, Xu Chen and Jian Huang

Antisense oligonucleotide-mediated chimeric RNA knockdown / Anam Tajammal, Katie Do and Hui Li

Knockdown of chimeric RNA by RNAi / Liang Cheng, XiaoLe Lu, Chenwei Yang, Xu Chen and Jian Huang

Bioinformatic discovery of tumor-specific neoantigens arising from chimeric RNAs / Samir Lalani, Sehajroop Gadh and Hui Li

Chimeric RNAs as novel neoantigens for extracellular vesicles-based vaccines against cancer / Mengyuan Hu, Xiaona Lin, Huiying Huang, Yuhua Meng and Hao Zhang

A sex-specific chimeric RNA in female immune regulation : Case study / Xinrui Shi, Loryn Blackburn, Sandeep Singh, Martyna Glowczyk-Gluc, Shafaque Zahra, Peng Wu and Hui Li

Fusion RNA profiling in cancer : Investigating the etiology of fusion-gene-associated cancers and proposing an approach to study connections between biological samples / Zhongqiu Xie, Huiling Yuan and Hui Li

Discovery of a polymorphic gene fusion via bottom-up chimeric RNA prediction : Case study / Yiwei Wang, Justin Elfman and Hui Li

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed July 13, 2026)

Other Format:

Print version: Chimeric RNAs

ISBN:

9781071652022

1071652028

OCLC:

1602637681

Publisher Number:

CIPO000366808

Access Restriction:

Restricted for use by site license