Biomolecular display technologies / edited by Huan Peng, Theam Soon Lim

Format:

Book

Contributor:

Peng, Huan (Biomedical engineer), editor.

Lim, Theam Soon, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; 3030.

Methods in molecular biology, 1940-6029 ; 3030

Language:

English

Subjects (All):

Protein engineering.

Peptides--Biotechnology.

Peptides.

Protein Engineering.

Medical Subjects:

Protein Engineering.

Physical Description:

1 online resource : illustrations

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume explores major biomolecular display platforms and their applications, which allow researchers to mine vast, diverse libraries for functional variants, bridging molecular design and real-world application. Beginning with a section on foundational techniques for functional libraries, the book continues by covering tailored yeast, phage, and cDNA display strategies to boost efficiency, modulate binding, and engineer scaffolds for specialized needs, display-driven identification of macrocyclic ligands, nanoparticle-binding peptides, epitopes, and functional proteins, as well as computational advances, protein engineering, and post-selection analysis to refine hits and accelerate translation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Biomolecular Display Technologies offers expert guidance and insight into the transformative potential of display technologies"-- Springer Nature Link

Contents:

Incorporation of butyryl-lysine into phage-displayed peptide libraries / Sophea Pa, Rutendo Nyamadzawo, Gopal Dubey, J. Trae Hampton and Wenshe R. Liu

Construction and functional validation of a high-diversity naive phage antibody library / Angela Chiew Wen Ch’ng, Azimah Ahmad, Yee Siew Choong and Theam Soon Lim

Construction of a cyclic peptide library for phage display / Nicole Pinnette and Fa-Jie Chen

Generation of a CDR H3 randomized monoclonal antibody library by Kunkel mutagenesis / Jia Xuan Yeoh, Yee Siew Choong and Theam Soon Lim

Yeast display of single-chain Fc fragments with modulated binding of effector molecules / Katharina Stadlbauer, Cecilia Wick, Julia Gobl, Wolfgang Wallner, Annika de Sousa Linhares, Jochen Stritzker and Gordana Wozniak-Knopp

A simple and rapid method for preparing VHH antibody-template DNA conjugates for loop-mediated isothermal amplification (LAMP) method from cDNA display / Junpei Yamashita and Naoto Nemoto

M13 bacteriophage pVIII protein : Display strategies, chemical modification, and antibacterial activity investigations / Xuanliang Wang, Zongyuan Fan, Ziyue Huang, Zishan Wang, Zhijie Hu, Dairong Li and Huan Peng

Yeast molecular display for the screening and production of immunological proteins using nanobody as an example / Seiji Shibasaki, Wataru Aoki and Mitsuyoshi Ueda

Application of phage-displayed peptide library for epitope mapping / Belle Li Ling, Angela Chiew Wen Ch’ng, Yee Siew Choong and Theam Soon Lim

Phage display biopanning strategy for discovery of nanoparticle-binding peptides / Piotr Golec and Grzegorz Wegrzyn

De novo discovery of prenylated macrocyclic peptide ligands / Sota Uchida, Hiroaki Suga and Yuki Goto

Methods for the in vitro selection of protein and peptide libraries using mRNA display / Colin M. Leaf, Pearl Qi, Richard W. Roberts and Terry T. Takahashi

AI-driven protein research : From prediction to design / Guodong Min and Huan Peng

Engineering and adapting disulfide-containing proteins to enable intracellular functionality / Elise Sylvander and Michael W. Traxlmayr

High-performance computing in tandem mass spectrometry (MS/MS) peptide identification / Chuang Li, Qiang He, Tao Chen and Yanhua Wen

Post-selection methods for analyzing mRNA display selections and optimization of hits / Pearl Qi, Colin M. Leaf, Richard W. Roberts and Terry T. Takahashi

Tracking synthetic adhesins on bacterial surfaces with immunofluorescence microscopy / Sofía Fraile, Jaime Fernández de Córdoba, Víctor de Lorenzo and Esteban Martínez-García

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed July 13, 2026)

ISBN:

9781071652497

1071652494

OCLC:

1602637873

Access Restriction:

Restricted for use by site license