Metabolic gene clusters : methods and protocols / edited by Hans-Wilhelm Nützmann, Ramesha Thimmappa

Format:

Book

Contributor:

Nützmann, Hans-Wilhelm, 1982- editor.

Thimmappa, Ramesha, editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 3054.

Methods in molecular biology, 1940-6029 ; 3054

Language:

English

Subjects (All):

Genomics.

Medical Subjects:

Genomics.

Physical Description:

1 online resource : illustrations

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume provides a comprehensive guide of cutting-edge technologies applied in the discovery of metabolic gene clusters across different domains of life. Chapter guide readers through cluster identification, characterization, evolutionary analyses, synthetic assembly, pathway re-constitution, cluster regulation and activation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Metabolic Gene Clusters: Methods and Protocols aims to spur new discoveries in the field of metabolic gene clusters and bring different areas of research closer together"-- Springer Nature Link

Contents:

Generalized gene cluster detection using CLOCI / Zachary Konkel and Jason Slot

In silico identification and comparative synteny of biosynthetic gene clusters in plants / Song Wu and Evangelos C. Tatsis

Mining metabolic diversities across genomic neighborhoods in plants / Xuan Zhou and Zhenhua Liu

Identification of stress-induced biosynthetic gene clusters in plants / Amir Ronen, Boaz Negin, Michal Rosenthal, and Guy Polturak

Investigating intraspecific diversity of metabolic gene clusters with MLPA and synthetic probes / Viktoriia Plyhun, Anastasiia Satyr, and Agnieszka Zmienko

Synteny analysis and visualization of gene clusters / Chanchan Liu, Dishuai Li, and Qinan Wu

Automating plasmid construction for high-throughput heterologous expression of fungal gene clusters / Anjali D. Shah, Andrew M. Bailey, and Katherine Williams

Molecular lego in the green factory : engineering plant metabolism with Golden Gate assembly / Rajesh Chandra Misra, Ramesha Thimmappa, and Anne Osbourn

Methods for functional validation of terpenoid metabolic clusters in Nicotiana benthamiana and Aspergillus oryzae / Zheyong Xue, Chang Li, Xue Yin, Jiaojiao Lu, and Yanhong Guo

Characterization of bioactive saponins from sea cucumbers / Karuna Bhaskaran, Rajesh Chandra Misra, Anne Osbourn, and Ramesha Thimmappa

Rhizobium rhizogenes-mediated hairy root transformation protocol for Lotus japonicus and other legumes / Panagiotis Vletsos, Jenny Koukara, and Kalliope K. Papadopoulou

CUT&Tag epigenomic profiling of biosynthetic gene clusters in Arabidopsis thaliana / Jessica Taylor, Kieran Reynolds, and Hans‑Wilhelm Nützmann

Mapping the 3D chromosome organization of Streptomyces by Hi-C / Zhiyu Zhong, Lin Liu, Liang Deng, Zhihu Zhao, and Yuhui Sun

Mapping the 3D chromosome organization of a biosynthetic gene cluster by capture Hi-C (CHi-C) / George Jervis and Hans‑Wilhelm Nützmann

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed July 8, 2026)

Other Format:

Print version: Metabolic gene clusters

ISBN:

9781071653845

1071653849

OCLC:

1602637707

Access Restriction:

Restricted for use by site license