Genome editing for precision crop breeding / edited by Matthew Willmann.

Format:

Book

Contributor:

Willmann, Matthew, editor.

Series:

Burleigh Dodds Series in Agricultural Science

Language:

English

Subjects (All):

Plant breeding.

Physical Description:

1 online resource (495 pages) : illustrations

Edition:

1st ed.

Place of Publication:

Philadelphia, Pennsylvania : Burleigh Dodds Science Publishing, [2021]

Summary:

"Genome editing is rapidly transforming plant research. The technique offers unparalleled precision in breeding without the need to introduce foreign DNA into plants. CRISPR/CAS systems have established themselves as the leading technique in genome editing. This collection takes stock of the wealth of research on these techniques and their potential in crop breeding in improving traits such as yield, disease resistance, drought tolerance and nutrient use efficiency. Part 1 of this volume reviews advances in gene editing techniques such as TALENS and zinc finger nucleases, double-strand break repair techniques, insertion-based genome edits, base editing, guide RNAs, gRNA/Cas9 constructs and CRIST/CAS off targeting. Chapters also discuss advances in screening plants as well as regulatory issues. Part 2 surveys applications of gene editing in key cereal including wheat, barley, maize, rice and sorghum. Other chapters cover crops such brassicas, potato and tomato"-- Provided by publisher.

Contents:

Cover

Half Title

Series Page

Title Page

Copyright Page

Contents

Series list

Acknowledgements

Introduction

Part 1 Genome editing techniques

1 Using TALENs for genome editing in plants

1 Introduction

2 How TALENs work

3 How have TALENs been used?

4 How to use TALENs

5 Conclusion

6 Acknowledgements

7 References

2 Double strand break (DSB) repair pathways in plants and their application in genome engineering

2 DSB repair via non-homologous end joining

3 DSB repair using homologous sequences

4 Perspectives

5 Where to look for further information

6 References

3 Advances in the generation of insertion-based genome edits in plants

2 Outcomes of knock-ins

3 The manner of targeting the integration of DNA fragments

4 Methods to increase the efficacy and accuracy of knock-ins

5 Conclusion and future trends

6 Where to look for further information

4 Viruses as vectors for the delivery of gene-editing reagents

2 DNA viruses: replicon vectors for efficient gene editing through homology-directed repair (HDR)

3 Geminiviral replicons (GVRs): deconstructed geminiviruses that serve as replicating vectors in plants

4 Replicon vectors for efficient homology-directed repair

5 Case studies: GVR-based editing strategies in dicots and monocots

6 GVR-based editing strategies: summary and future trends

7 RNA viruses: mobile vectors broadly applicable for gene-editing reagent delivery

8 Case studies: use viral vectors to create permanent genetic changes

9 RNA viral vectors: summary and future trends

10 Where to look for further information

11 References

5 Progress in precise and predictable genome editing in plants with base editing

2 Progress in mammalian systems.

3 Cytosine base editing in plants

4 Adenine base editing in plants

5 Broadening protospacer adjacent motif (PAM) accessibility

6 Sequence diversification in crops

7 Off-target base editing

8 Current applications of base editors in crops

9 Conclusion

6 Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems

2 Designing sgRNAs

3 Bioinformatics programs for designing sgRNAs

4 Predicting the outcome of CRISPR-mediated genome editing

5 Conclusions

7 Advances in assembling gRNA/Cas9 constructs in genome editing of plants

2 Modular cloning tools for assembling CRISPR/Cas constructs

3 The basics of CRISPR/Cas9 cloning

4 CRISPR-Cas12 specific cloning requirements

5 Expanding CRISPR/Cas9 activities with modular cloning

6 The polycistronic strategy for gRNA multiplexing

7 Case study: multiple gene targeting in tobacco with GoldenBraid cloning

8 References

8 Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants

2 Delivery of genome editing components into plant cells

3 Delivery methods for genome editing reagents: delivery into single cells

4 Delivery methods for genome editing reagents: delivery into intact tissues

5 Alternatives to DNA delivery

6 Morphogenic genes increase transformation efficiency and extend genotype range

7 Morphogenic genes permit transformation in nontraditional explants

8 Future trends

9 Where to look for further information

10 References

9 Advances in screening plants for edits and off-targets

2 PCR/RE assay

3 Mismatch cleavage assay

4 PCR/RNP.

5 High-resolution melting (HRM) analysis

6 ACT-PCR

7 Analysis of Sanger sequencing chromatograms

8 Next generation sequencing (NGS)

9 Detection of off-target effects

10 Summary and future trends

11 Where to look for further information

12 References

10 Targeted modification of promoters

2 Plant promoters, cis-acting regulatory elements and leading introns

3 Principles of targeted promoter modification

4 Approaches for targeted promoter modification

11 The regulation of genome-edited crops

2 Background on genome editing and its regulation

3 Whether the Cartagena Biosafety Protocol applies to genome-edited crops

4 Country case studies demonstrating regulation of genome-edited crops

5 Analysis of regulations in different countries

6 Conclusion and future trends

Part 2 Applications

12 Genome editing of barley

2 Expression systems used for targeted mutagenesis

3 Enhancing plant development and performance

4 Molecular understanding of resistance against plant pathogens

5 Targeted mutagenesis for molecular farming purposes

6 Conclusion

7 Where to look for further information

13 Genome editing of maize

2 Site-specific engineering in maize: an overview

3 Current challenges in maize genome editing

4 Case study: gene editing of a recalcitrant line by co-bombardment of morphogenic genes (Babyboom/Wuschel) and CRISPR/Cas9 reagents

14 Genome editing in sorghum

2 Advances of gene editing in sorghum.

3 Application of CRISPR/Cas9 gene editing for sorghum protein improvement

4 Future trends

15 CRISPR/Cas9-mediated genome editing in Brassica

2 Gene disruption in Brassica species

3 Homologous recombination-based editing in Brassica species

4 Case study: generating mutants by CRISPR/Cas9 toolbox

16 Genome editing of tomatoes and other Solanaceae

2 Gene editing proof-of-concept investigations

3 DNA sequence insertion

4 Application of CRISPR/Cas ribonucleoprotein complexes

5 Base editing

6 Modification of gene function for improved disease tolerance

7 Editing to affect fruit and tuber quality

8 Plant growth and development modifications

9 Case study: application of gene editing for de novo domestication

10 Conclusion and future trends

17 Genome editing of woody perennial trees

2 The heterozygous genome challenge in gene editing

3 Genome resource limitation of woody perennial crops

4 Gene-editing reagents

5 Stability

6 CRISPR/Cas9-empowered characterization of duplicated genes

7 Conclusion and future trends

8 Where to look for further information

9 Acknowledgements

Index.

Notes:

Description based on print version record.

ISBN:

1-78676-450-4

1-78676-449-0

OCLC:

1246578909