Mouse embryogenesis methods and protocols edited by Paul Delgado-Olguín

Format:

Book

Contributor:

Delgado-Olguin, Paul, editor.

Series:

Methods in molecular biology ; 3010.

Springer protocols (Series)

Methods in molecular biology 1940-6029 3010

Springer protocols

Language:

English

Subjects (All):

Mice--Embryology.

Mice.

Mice--embryology.

Medical Subjects:

Mice--embryology.

Genre:

Laboratory manuals

Laboratory manuals.

Physical Description:

1 online resource

Edition:

Second edition

Place of Publication:

New York, NY Humana Press [2026]

Summary:

"This second edition volume expands on the previous edition with new methods and refinements ranging from tissue clearing and three-dimensional imaging, to explant culture systems, lineage tracing, high-resolution transcriptomics, metabolism, and chromatin structure analysis. The chapters in this book are organized by molecular depth, progressing from foundational methods to approaches that probe cellular and nuclear mechanisms. The protocols presented here reveal how cellular behaviors and molecular pathways are coordinated to shape the embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mouse Embryogenesis: Methods and Protocols, Second Edition is a valuable resource for both expert and novice researchers who are looking to learn more about this advancing field. The methods discussed in this volume will inspire new questions and experimental strategies to further advance our understanding of mammalian development"-- Springer Nature Link

Contents:

Mouse genotyping / Yunhan Li, Sandra Vuong, Lijun Chi, and Paul Delgado-Olguín

Dynamic imaging of mouse embryos and cardiodynamics in static culture / Andrew L. Lopez III and Irina V. Larina

3D imaging of gene expression domains in the mouse heart / Wataru Katano, Yuki Tajika, and Kazuko Koshiba-Takeuchi

Fetal mouse heart clearing, imaging, and 3D reconstruction / Hamna Ammar and Paul Delgado-Olguín

Visualization of the embryonic great vessels using plastic resin injection / Rimshah Abid, Yena Oh, and Kyoung-Han Kim

Explant culture for studying lung development / Behzad Yeganeh and Martin Post

Embryonic gut explant model for enteric neural crest cells migration / Dorothy Lee and Agostino Pierro

Embryonic gut enteric neural crest cells-derived neurospheres / Dorothy Lee, Naho Fujiwara, and Agostino Pierro

Preparation of mouse embryonic eye sections for routine histology / Esmeralda Lira-Romero, Francisco Javier Estrada-Mena, and Israel Ramírez-Sánchez

Embryonic mouse cardiac fibroblast isolation / Enrique Mendez-Bolaina, Elena de la Cruz Herrera-Cogco, and Israel Ramírez-Sánchez

Standardized isolation, culture, and imaging of contracting cardiomyocytes from neonatal mouse hearts / Jielin Yang, Miranda Pannella, and Paul Delgado-Olguín

Isolation and culture of neonatal mouse atrial and ventricular myocytes / Yena Oh, Julie Pan, and Kyoung-Han Kim

MicroRNA quantification in fast- and slow-twitch skeletal muscles of mouse / Magally Ramírez-Ramírez, María Luisa Benítez-Hess, Estela G. García-González, and J. Manuel Hernández-Hernández

Gene expression analysis on single myofibers from mouse EDL and soleus muscles / Amairani Cancino-Bello, Mauricio Hernández-Somilleda, Estela G. García-González, and J. Manuel Hernández-Hernández

Characterization of adipocyte precursor cells (APCs) by lineage tracing, flow cytometry, and cytometry time of flight (CyTOF) / Qiwei Zhang, Nathaniel Vo, Ju Hee Lee, Azadeh Teyganeh, Kafi Ealey, and Hoon-Ki Sung

Investigating protein interactions in single cells by proximity ligation assay (PLA) / Zoe E. Gillespie, Saloni Modi, Mariia Cherednychenko, Mario Felice, Navroop K. Dhaliwal, and Jennifer A. Mitchell

Metabolic plasticity in embryogenesis throughout the lens of NAD+ / Quetzalcoatl Escalante-Covarrubias and Paul Delgado-Olguín

3D chromatin architecture during early development : new methods and new findings / Eduardo Blanco-Olías, Karina Jácome-López, Rosario Pérez-Molina, and Mayra Furlan-Magaril

Notes:

1st edition published in 2018

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed May 28, 2026)

Other Format:

Print version Mouse embryogenesis

ISBN:

9781071651261

1071651269

OCLC:

1592831322

Access Restriction:

Restricted for use by site license