Lipid-protein interactions : methods and protocols

Format:

Book

Contributor:

Kleinschmidt, Jörg H., editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 3001.

Springer protocols (Series)

Methods in Molecular Biology, 1940-6029 ; 3001

Springer protocols

Language:

English

Subjects (All):

Lipoproteins.

Membrane lipids.

Membrane proteins.

Protein binding.

Membrane Lipids.

Membrane Proteins.

Protein Binding.

Medical Subjects:

Lipoproteins.

Membrane Lipids.

Membrane Proteins.

Protein Binding.

Physical Description:

1 online resource (xvi, 932 pages) : illustrations.

Edition:

Third edition / edited by Jörg H. Kleinschmidt

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This third edition expands upon the chapters of the previous editions, providing new and updated methods. Chapters explore protein-lipid interactions, membranes, membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer, and lipid properties. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Lipid-Protein Interactions: Methods and Protocols, Third Edition is an essential resource for all researchers who are interested in obtaining up-to-date and comprehensive information about membrane structure and function"-- Springer Nature Link

Contents:

Characterization of membrane binding and protein–lipid interactions at the atomic level with an accelerated HMMM model / Hale S. Hashemi, Rupeng Li, Payam Helchi, Po-Chao Wen and Emad Tajkhorshid

Quartz crystal microbalances as tools for probing protein–membrane interactions / Søren B. Nielsen and Daniel E. Otzen

Surface plasmon resonance for measuring interactions of proteins with lipids and lipid membranes / Aleksandra Šakanovic, Vesna Hodnik, Apolonija Bedina Zavec, Kristina Eleršic Filipic and Gregor Anderluh

Isothermal titration calorimetry for investigating thermodynamics of protein–lipid interactions / Musti J. Swamy and Sonali S. Pawar

Differential scanning calorimetry of protein–lipid interactions / Olga Cañadas and Cristina Casals

Purification of bacterial lipopolysaccharide, and how to study its interactions with proteins / Athanasios Saragliadis, Daniel Hatiem and Dirk Linke

Analysis of the kinetics of folding and insertion of ß-barrel membrane proteins by gel-electrophoresis / Jennifer Hoppe, Agnes Maršál, Sascha Herwig, Andre Schüßler and Jörg H. Kleinschmidt

Imaging lipid-associated macromolecular structures by optimized negative-staining transmission electron microscopy / Jianfang Liu, Meng Zhang and Gang Ren

Probing the annular lipid belt of membrane proteins using native mass spectrometry / Swiriti Kumar and Arthur Laganowsky

Protein microarrays and liposome: a method for studying lipid–protein interactions / Aaron Chen, Samuel Heriantao, Chien-Sheng Chen and Heng Zhu

Complex structural examination of protein–lipid interactions with neutron scattering techniques / Najet Mahmoudi, Hannah Johnston, Sophie E. Ayscough, Stephen Hall, Hanna Wacklin-Knecht, Timothy J. Knowles, Nicolo Paracini, Marit Cardenas, Gerhard Gröbner, Jeremy Lakey, Frank Heinrich and Luke A. Clifton

Circular dichroism and synchrotron-radiation circular dichroism spectroscopy as tools to monitor protein structure in a membrane environment / Koichi Matsuo and Kunihiko Gekko

Protein–membrane interactions studied by transmission and total internal reflection FTIR spectroscopy / Surem A. Taufienan

Deep UV resonance Raman spectroscopy: applications to membrane proteins and peptides / Chuanmei B. Tanjiranasaree, Donovan G. Rix and Yujie E. Kim

Analyzing transmembrane protein and hydrophobic helix topography by dual fluorescence quenching / Gregory A. Caputo and Erwin London

Förster resonance energy transfer as a tool for quantification of protein–lipid selectivity / Luis M. S. Loura, Manuel Prieto and Fábio Fernandes

A guide to tracking single membrane proteins and their interaction in supported lipid bilayers / Evan L. Taylor, Kumuda Raj Foudel and James A. Brock

Fluorescence correlation spectroscopy to examine protein–lipid interactions in membranes / Victoria Betaneli, Jan-Hagen Kropp, Jonas Mickschel and Petra Schwille

Membrane pore formation by peptides studied by fluorescence techniques / Suren A. Tatulian and Nabin Kandel

Site-directed fluorescence spectroscopy for studies on the folding and insertion of proteins in lipid bilayers and model membranes / Líy Nguye, Sacha Herwig, Lisa Gerlach, Sara Gholami, Nicole Schürmann and Jörg H. Kleinschmidt

EPR techniques to probe insertion and conformation of spin-labeled proteins in lipid bilayers / Enrica Bordignon, Svetlana Kucher and Kevan Pol

Electron paramagnetic resonance spectroscopy of spin-labeled lipids for studying lipid–protein interaction in model and biomembranes / Tibor Páli and Zoltán Kóta

Non-linear continuous wave electron paramagnetic resonance spectroscopy for investigation of lipid–protein interactions in membranes / Tibor Páli

Solid-state NMR approaches to study protein structure and protein–lipid interactions in bulk and heterogeneous membranes / Christopher Aisenbrey, Evgeniy S. Salnikov, Jesus Raya and Matthias Michalek

Solution NMR spectroscopy for the determination of structures of membrane proteins in a lipid environment / Runnya Pandey and Ashish Arora

The nanodiscs system for investigating protein–lipid interactions / Ilia G. Denisov and Stephen G. Sligar

Measurement of the refractive index of monomolecular layers in Brewster angle microscopy to study lipid–protein layers at the air–water interface / Julio M. Pusterla, Jean M. Vargas Fondacaro and Guillermo G. Montich

Investigating peptide–lipid membrane interactions using small angle X-ray and neutron scattering / Vladimir Roseno Kornyakov, Kari Kristine Almsrod and Reidar Lund

A comprehensive approach to monitor endocytosis and trafficking of G protein-coupled receptors: tools for exploring receptor–lipid interaction / Mathan Sharma and Amitabha Chattopadhyay

Mitochondrial transmembrane ß-barrels: deducing thermodynamic regulators of folding and stability / Ulf Kummer, Tanja Kaminska and Ruchira S. Mahalakshmi

Determining the hydrodynamic size of lipid nanoparticles by fluorescent universal lipid labeling for microfluidic diffusion sizing (FULL-MDS) / Loretta Pienczewski, Lena Bauerhofer and Sandro Keller

Multiscale simulation approaches to probe lipid interactions of protein-coupled receptors / Durba Sengupta, Shalmali Kharche, Aditya Manoj, Amit Naglekar and Amitabha Chattopadhyay

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed June 10, 2026)

Other Format:

Print version: Lipid-protein interactions

ISBN:

9781071650547

1071650548

OCLC:

1594330066

Publisher Number:

CIPO000353330

Access Restriction:

Restricted for use by site license