Molecular dermatology : methods and protocols / edited by Natalia V. Botchkareva, Gillian E. Westgate

Format:

Book

Contributor:

Botchkareva, Natalia V., editor.

Westgate, Gillian E., editor.

Series:

Methods in molecular biology ; v. 3031.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3031

Springer protocols

Language:

English

Subjects (All):

Skin--Diseases--Molecular aspects.

Skin.

Dermatology, Experimental.

Skin Diseases--pathology.

Pathology, Molecular--methods.

Molecular Diagnostic Techniques--methods.

Skin--Diseases.

Medical Subjects:

Skin Diseases--pathology.

Pathology, Molecular--methods.

Molecular Diagnostic Techniques--methods.

Genre:

Laboratory manuals

Laboratory manuals.

Manuales de laboratorio

Physical Description:

1 online resource : illustrations (some color)

Edition:

Second edition

Place of Publication:

New York : Humana Press, [2026]

Summary:

"This second edition volume expands on the previous edition with a discussion on the latest advancements in laboratory techniques used in dermatological research. The chapters in this book cover topics such as methodologies for establishing 2D and 3D skin models; assessing DNA damage; genome editing using CRISPR/Cas systems; analyzing extracellular vesicles involved in intercellular communication; and detecting microflora and skin permeability barrier. This book also presents experimental strategies for modeling and modulating cutaneous pathologies, including wound healing and its associated microbiome, skin cancer, and hair follicle disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Molecular Dermatology: Methods and Protocols, Second Editon is a valuable resource for researchers working in both fundamental and applied dermatological science"-- Springer Nature Link

Contents:

Isolation of epidermal keratinocytes from human skin : the scratch-wound assay for the assessment of epidermal keratinocyte migration / Stephen K. Sikkink, Irene Castellano‑Pellicena, and M. Julie Thornton

Isolation of different dermal fibroblast populations from the skin and the hair follicle / Rachael Williams and M. Julie Thornton

Isolation of epidermal and hair follicle melanocytes / Richard Baker and M. Julie Thornton

Validated step-by-step protocols for human keratinocyte isolation and the construction of organotypic 3D epidermal models / Jaimy A. Klijnhout, Rens H. W. Peters, Gijs Rikken, Diana Rodijk‑Olthuis, Sandrine Dubrac, Yves Poumay, Hanna Niehues, Jos P. H. Smits, and Ellen H. van den Bogaard

Human epidermal equivalents on nonwoven scaffolds : a model of tissue formation in an artificial environment / Elena V. Sytina, Olga A. Romanova, and Andrey A. Panteleyev

Purification of extracellular vesicles secreted by skin fibroblasts and keratinocytes / Jérôme Lamartine and Isabelle Petit

Genetic engineering of human keratinocytes using CRISPR/Cas9 ribonucleoprotein complexes or modified Cas9‑encoding mRNAs / Jaimy A. Klijnhout, Eline A. W. Senders, Ellen H. van den Bogaard, and Jos P. H. Smits

Imaging approach to DNA damage induction and quantification / Mikael V. Garabedian

Identification of long noncoding RNA by in situ hybridization approaches / Mara Mancini, Anna Maria Lena, and Eleonora Candi

Detection of microRNAs in skin by in situ hybridization / Maximilian E. Pickup and Mohammed I. Ahmed

3D‑FISH analysis of the spatial genome organization in mammalian tissue in situ / Andrei N. Mardaryev and Michael Y. Fessing

Methods to investigate the epidermal permeability barrier and the light irradiation barrier in vitro and ex vivo / Yoshikazu Uchida and Anna Celli

A protocol to prepare and stain dorsal skin whole mounts from mice / Natalia A. Veniaminova, Marina Grachtchouk, Andrzej A. Dlugosz, and Sunny Y. Wong

Isolation of mouse hair matrix keratinocytes for multi‑omics analysis / Guodong Chen, Elena Rozhkova, and Andrey A. Sharov

Methods to study human hair follicle growth ex vivo : human microdissected hair follicle and human full‑thickness skin organ culture / Janin Edelkamp and Marta Bertolini

Generation of hair follicle germs in vitro using human postnatal skin cells / Ekaterina P. Kalabusheva and Ekaterina A. Vorotelyak

In situ laser‑capture microdissection for detection of components of the hair follicle and scalp microbiome / Janin Edelkamp and Marta Bispo Lousada

Viable vs. nonviable microbiota evaluation of the hair follicle and scalp microbiome / Janin Edelkamp and Marta Bispo Lousada

Nonsurgical induction of alopecia areata in C3H/HeJ mice via adoptive transfer of cultured lymphoid cells / Eddy H. C. Wang and Kevin J. McElwee

Collapse and restoration of hair follicle immune privilege ex vivo : a model for alopecia areata / Markus Fehrholz and Marta Bertolini

Experimentally induced epithelial–mesenchymal transition of human hair follicle stem cells as a model of scarring alopecia ex vivo / Ilaria Piccini and Marta Bertolini

Method for human sweat gland isolation from the scalp and axilla by means of the micro‑punch FUE technique and vital dyes / Francisco Jimenez and Marie‑Andrée Schambach

Assessment of acute wound healing using excisional mouse tail skin wound models / Iqra Fatima and Andrey A. Sharov

Skin and wound infection models to test host–pathogen interactions and evaluate topical antimicrobial treatments / Franziska Schwarz, Christin Tautz, Andreas Nitsche, Marica Grossegesse, Annika Vogt, Ulrike Blume‑Peytavi, Christoph Schaudinn, and Fiorenza Rancan

Human wound healing ex vivo model with focus on molecular markers / Ilaria Piccini, Janin Edelkamp, and Marta Bertolini

The evaluation of fibrotic alterations in regenerated murine and human xenografted skin / Olga L. Cherkashina, Olga S. Rogovaya, Danila S. Abolin, Elena I. Morgun, Ekaterina A. Vorotelyak, and Ekaterina P. Kalabusheva

Method to study skin cancer : two‑stage chemically induced carcinogenesis in mouse skin / Andrei N. Mardaryev, Iqra Fatima, and Andrey A. Sharov

Ultraviolet irradiation of skin cells / Thomas M. Rünger

Method for investigation of photobiological effects of light on human skin cells in vitro using visible and near‑infrared light / Charles‑Antoine Georges Marie Mignon and Natallia Eduardauna Uzunbajakava

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed June 4, 2026)

Other Format:

Print version: Molecular dermatology

ISBN:

9781071652534

1071652532

OCLC:

1592132247

Publisher Number:

CIPO000382317

Access Restriction:

Restricted for use by site license