Macrophage migration inhibitory factor : methods and protocols / edited by James Harris, Timothy W. Muusse

Format:

Book

Contributor:

Harris, James (Immunologist), editor.

Muusse, Timothy W., editor.

Series:

Methods in molecular biology (Clifton, N.J.) ; v. 3002.

Springer protocols (Series)

Methods in molecular biology, 1940-6029 ; 3002

Springer protocols

Language:

English

Subjects (All):

Macrophage migration inhibitory factor.

Immunology.

Diseases--Animal models.

Diseases.

Macrophage Migration-Inhibitory Factors--immunology.

Macrophage Migration-Inhibitory Factors--analysis.

Macrophage Migration-Inhibitory Factors--therapeutic use.

Disease Models, Animal.

Macrophage Migration-Inhibitory Factors.

Medical Subjects:

Macrophage Migration-Inhibitory Factors--immunology.

Macrophage Migration-Inhibitory Factors--analysis.

Macrophage Migration-Inhibitory Factors--therapeutic use.

Disease Models, Animal.

Macrophage Migration-Inhibitory Factors.

Genre:

Laboratory manuals.

Physical Description:

1 online resource (xii, 296 pages) : illustrations

Edition:

Second edition

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This second edition volume expands on the previous edition with new protocols that offer biochemical, biophysical, and biological assays for studying macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (D-DT). The chapters in this book cover topics such as generation of MIF peptides via solid phase peptide synthesis; fragment screening of MIF by surface plasmon resonance; MIF NMR chemical shift perturbation mapping; methods to determine the role of MIF in cilia biogenesis; and immunofluorescent quantification of MIF in larval zebrafish tailfin transfection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Macrophage Migration Inhibitory Factor: Methods and Protocols, Second Edition is a valuable resource for any researcher hoping to contribute to the ever expanding world of MIF biology"-- Springer Nature Link

Contents:

Expression and purification of recombinant macrophage migration inhibitory factor (MIF) / Samie Elmazi, Emmanuel K. Yeboah, Stephen J. Headey, and Natalie A. Borg

Generation of MIF peptides via solid phase peptide synthesis / Aliyah Veronica R. Pilien, Polina Eidelberg, Yadwinder S. Mann, Georgios Pantouris, and Jianhua Ren

Expression and purification of recombinant D‑dopachrome tautomerase (D‑DT) / Emmanuel K. Yeboah, Samie Elmazi, Stephen J. Headey, and Natalie A. Borg

Structural determination and ligand interaction of macrophage inhibitory factor : a standardized X‑ray crystallography approach / Emmanuel K. Yeboah and Alessandro T. Caputo

Generation of high‑quality D‑dopachrome tautomerase crystals for structural studies / Christopher Argueta and Georgios Pantouris

Fragment screening of MIF by surface plasmon resonance / Emmanuel K. Yeboah, Natalie A. Borg, and Stephen J. Headey

Detection of noncovalent protein–ligand complexes of D‑DT variants by MALDI‑TOF mass spectrometry / Andrew Parkins, Georgios Pantouris, and Andreas H. Franz

Analysis of D‑dopachrome tautomerase conformational flexibility through molecular dynamics simulations / Andrew Parkins, Christopher Argueta, and Georgios Pantouris

MIF NMR chemical shift perturbation mapping / Emmanuel K. Yeboah, Natalie A. Borg, and Stephen J. Headey

Assessing the tautomerase activity of MIF using L‑dopachrome / Katie Treasure, Amanda Genders, and James Harris

Using 4‑HPP and PP as substrates to assess purified MIF and D‑DT tautomerase activity and inhibition / Emmanuel K. Yeboah, Samie Elmazi, Stephen J. Headey, and Natalie A. Borg

Protocols for assessing the nuclease activity of MIF / Longfei Li, Seo‑Hyun Kim, Ted M. Dawson, and Valina L. Dawson

Detection of oxidized macrophage migration inhibitory factor (oxMIF) in biological samples using enzyme‑linked immunosorbent assay (ELISA) / Gregor Rossmueller and Michael Thiele

Detection of oxidized macrophage migration inhibitory factor (oxMIF) in tissue samples using immunohistochemistry (IHC) / Alexander Schinagl

Exploring the chemotactic effects of MIF on neutrophils and T cells and methods for selective receptor targeting / Zhishen Zhang, Aphrodite Kapurniotu, Jürgen Bernhagen, and Adrian Hoffmann

Polarization of bone marrow‑derived macrophages from wild‑type and high human MIF‑expressing CATT7 polymorphic mice / Molly Dunlop, Sinéad Ryan, Ian J. Hawthorne, Michelle E. Armstrong, Seamas C. Donnelly, and Karen English

Using luciferase reporter cells to assess the effects of MIF inhibitors on NF‑κB activation / Sarah Little, Katie Treasure, Srinivasan Jayashree, and James Harris

Monitoring inflammasome activity through ASC speck formation in THP‑1 ASC‑GFP cells / Timothy Muusse and Sarah Little

Small interfering RNA (siRNA)‑mediated knockdown of macrophage migration inhibitory factor transcript in adherent and suspension cultures of human cell lines / Swagata Ghosh, Mathan L, and Hari Vignesh Sekar

Stable transfection of CD74 gene variants into A549 lung adenocarcinoma cell line / Jasmine Vargas and Georgios Pantouris

Methods to determine the role of MIF in cilia biogenesis / Ewud Agborbesong and Xiaogang Li

Modeling fungal and amoebic infections : co‑culture approaches to study MIF’s role in infection‑induced inflammatory responses / Swagata Ghosh and Mathan L

Immunofluorescent quantification of MIF in larval zebrafish tailfin transection / Tracey Ollewagen and Carine Smith

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed June 4, 2026)

Other Format:

Print version: Macrophage migration inhibitory factor

ISBN:

9781071650561

1071650564

OCLC:

1593097337

Access Restriction:

Restricted for use by site license