Antibody engineering methods and protocols

Format:

Book

Contributor:

Nevoltris, Damien, editor.

Ollier, Romain, editor.

Series:

Methods in molecular biology ; v. 2998.

Springer protocols (Series)

Methods in molecular biology 1940-6029 2998

Springer protocols

Language:

English

Subjects (All):

Immunotechnology.

Immunoglobulins.

Antibodies--therapeutic use.

Antibodies--genetics.

Protein Engineering--methods.

Medical Subjects:

Antibodies--therapeutic use.

Antibodies--genetics.

Protein Engineering--methods.

Genre:

Laboratory manuals

Laboratory manuals.

Physical Description:

1 online resource

Edition:

Fourth edition edited by Damien Nevoltris, Romain Ollier

Place of Publication:

New York, NY Humana Press [2026]

Summary:

"This fourth edition volume expands on the previous editions by providing readers with a comprehensive guide to the latest foundational and advanced techniques used by researchers in the field. This book covers the construction of diverse antibody libraries including naïve, synthetic, and immune types and candidate selection using phage, yeast, or mammalian display systems. This book also discusses in silico approaches such as computational design, epitope prediction, and deep learning to improve antibody discovery and characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Antibody Engineering: Methods and Protocols, Fourth Edition is a valuable resource that provides scientists with an extensive toolbox to generate the next generation of antibody-based molecules for research, diagnostics, and therapeutic applications"-- Springer Nature Link

Contents:

IMGT nomenclature and IMGT unique numbering : The two pillars of 35 years of immunoinformatics for immunoglobulins (IG) or antibodies and T cell receptors (TR) / Marie-Paule Lefranc and Gérard Lefranc

Complementarity of deep learning and physics-based approaches in the design of new antibodies / Floriane Eshak and Anne Goupil-Lamy

Prediction of protein antigen epitopes from monoclonal antibodies by in silico molecular docking / Tânia Maria Manieri and Gabriela Massaro Barreiros

Antibody affinity maturation by computational design / Esam Tolba Abualrous, Wade Miller and Daniel Andrew Cannon

Construction of naïve and immune human Fab phage display library / Jia Xuan Yeoh, Jing Yi Lai, Angela Chiew Wen Ch’ng and Theam Soon Lim

Generation and panning of a naïve human scFv phage display library / Jia Xuan Yeoh, Jing Yi Lai, Angela Chiew Wen Ch’ng and Theam Soon Lim

Screening of monoclonal antibodies from integrated phage and mammalian cell display libraries / Diao Yu, Huixian Li, Yujia Jin, Yu Kong, Tianlei Ying, Yanling Wu and Chao Tu

In vitro selection of antibodies by ribosome display / He Huang, Xinran Gao and Guangbo Kang

Yeast display and competitive FACS : Powerful tools for the selection of rare clones from an scFv library / Yue Sun, Bhupal Ban and Diane A. Blake

Transient expression and characterization of antibodies and antibody fragments / Greg del Val, Florence Gauye, Romain Ollier and Damien Nevoltris

Updates to guidance on using Biacore for protein–protein interactions analysis / Hui Ma, Stephen Hearty, Paul Leonard and Richard O’Kennedy

Epitope binning : Clustering monoclonal antibodies on monomeric and multivalent antigens / Adva Mechaly and Ohad Mazor

High-throughput profiling of antibody-binding epitopes using AbMap / Wen-jing Du, Huan Qi and Sheng-ce Tao

Ultra-dilute developability analysis of antibody self-association and non-specific binding / Na-Young Kwon, Murat Karadag and Peter M. Tessier

Characterizing pH-dependent interactions between immunoglobulin and the neonatal Fc receptor using surface plasmon resonance / Sébastien Menant, Damien Nevoltris and Romain Ollier

Simultaneous affinity maturation and liability removal from antibodies / Camila Leal, Fortunato Ferrara, Andrew Raymon Morton Bradbury and Andre A. R. Teixeira

Antibody humanization / Tim D. Jones

Production of antibody fragments suitable for 1:1, site-specific labeling / Urša Štrancar and Ario de Marco

Combination of Fc-protein engineering strategies : Design and evaluation of antibody effector functions / Carina Lynn Gehlert, Steffen Krohn, Dorothee Winterberg, Ammelie Svea Boje, Thomas Theocharis, Alexander Jochimsen, Marta Lustig, Thomas Valerius, Christian Kellner, Katja Klausz and Matthias Peipp

Systematic production of IgG-like bispecific antibodies via intein-mediated protein trans-splicing / Hiroki Akiba and Kouhei Tsumoto

Generation and characterization of CAR-T cells / Sarah Ash, Greta Maria Paola Giordano Attianese, Paris Kosti, Aikaterini Semilietof, Evangelos Stefanidis, Melanie Triboulet and Melita Irving

Generation and characterization of bispecific VHH-derived surrogate agonists mimicking the function of IL-18 / Britta Lipinski, Desislava Elter, Paul Arras, Laura Unmuth, Simon Krah, Achim Doerner, Andreas Evers, Lukas Pekar and Stefan Zielonka

Antibody-drug conjugates synthesis using maleimide chemistry / Camille Martin and Sreenivasachary Nampally

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed May 21, 2026)

Other Format:

Print version: Antibody engineering

ISBN:

9781071650417

1071650416

OCLC:

1592101857

Access Restriction:

Restricted for use by site license