Bispecific antibodies : methods and protocols / edited by Masahiro Yasunaga

Format:

Book

Contributor:

Yasunaga, Masahiro, editor.

Series:

Springer protocols (Series)

Methods in molecular biology (Clifton, N.J.) ; v. 2997.

Methods in molecular biology, 1940-6029 ; 2997

Springer protocols

Language:

English

Subjects (All):

Bispecific antibodies.

Physical Description:

1 online resource (xii, 272 pages) : illustrations

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume explores protocols and perspectives for the design, production, characterization, functional evaluation, and clinical translation of bispecific antibodies (BsAbs). Beginning with a variety of platforms for generating BsAbs, the book continues with sections covering protocols to evaluate T-cell-dependent bispecific antibodies, modeling and simulation approaches to support clinical development of T-cell-engaging BsAbs, as well as clinical applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Bispecific Antibodies: Methods and Protocols aims to bridge the gap between molecular design and translational success by equipping investigators with the technical know-how and contextual understanding needed to show each method’s relevance to therapeutic development"-- Springer Nature Link

Contents:

Production of bispecific antibody combining knobs-into-holes and controlled fab-arm exchange methods / Leo Tern‑Cheng Lin and Kuen Ho Lee

Multimodal physicochemical characterization of cross-linking activities of bispecific antibodies / Hiroki Akiba, Koki Shiba, Kouhei Tsumoto, Hiroaki Ohno and Haruhiko Hamada

Generation of bispecific antibodies using fast-ig™ / Chien Ying Tso and Sine Feng

Imtac : a novel platform of T-cell receptor-based soluble bispecifics / Benjamin P. Oettinger and Aurelie Vidiot

Design and evaluation of domain-rearranged bispecific antibody fragments for functional optimization / Ryutaro Asano

Production of bispecific antibody fragments from E. fusion / Ryutaro Asano

Enhanced chemical method for bispecific antibody production : leveraging site-specific conjugation and stable crosslinking technology / Tomohiro Fujii and Yutaka Matsuda

Laboratory-scale production of recombinant IgG antibodies / Takahiro Amani and Masahiro Tsurafuga

Visualization of immunological synapse formation by intercellular cross-linking of T cell-dependent bispecific antibodies using fluorescence microscopy / Riikto Nakamura, Ryo Tsumura, Takahiro Amani, Ryotaro Asano and Masahiro Tsurafuga

Measurement of T cell migration by T cell-dependent bispecific antibody in cancer cells and T cell cross-linking / Riikto Nakamura, Ryo Tsumura, Takahiro Amani, Ryotaro Asano and Masahiro Tsurafuga

Optimized in vitro T cell exhaustion assay for evaluating T cell-dependent bispecific antibodies / Mayu Oka, Misako Hirasawaki and Masahiro Tsurafuga

Advanced evaluation of T cell-dependent bispecific antibodies using imaging and in vivo models / Mikiko Tsukakoshi and Manabu Yasuraga

Fluorescent-based analysis of T cells and T cell-dependent bispecific antibody in brain tumor mouse model / Yuri Nakanasawa, Teppei Asuma and Manabu Yasuraga

Peripheral blood mononuclear cell (PBMC) humanized mice as a platform for a preclinical in vivo evaluation of T cell-dependent bispecific antibodies / Ryo Tsumura, Aya Uchida and Masahiro Tsurafuga

Overview of quantitative clinical pharmacology for T cell engaging bispecific antibodies / Rong Deng and Chi‑Chung Li

Clinical development of T cell bispecific antibodies : review, challenges, and prospects solutions in solid tumor / Chika Ogami and Takahiro Ishiguro

Clinical development of bispecific antibodies : review, advances, and challenges in hematological malignancies / Anri Saitoh

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed June 16, 2026)

Other Format:

Print version: Bispecific antibodies

ISBN:

9781071650370

1071650378

OCLC:

1593104762

Publisher Number:

CIPO000353326

Access Restriction:

Restricted for use by site license