Bacterial efflux systems / edited by Ben Luisi.

Format:

Book

Contributor:

Luisi, Ben, editor.

Series:

Methods in enzymology ; 0076-6879 v. 724.

Methods in enzymology, 0076-6879 ; Volume 724

Language:

English

Subjects (All):

Bacteria.

Enzymology.

Physical Description:

xxviii, 533 pages : illustrations ; 24 cm.

Edition:

First edition.

Place of Publication:

Amsterdam : Academic Press, 2025.

Contents:

Preface

1. A robust protocol for refolding TolC and other outer membrane components of tripartite efflux pumps from inclusion bodies / Andrew Daufel, Andrés Cordova, S. Jimmy Budiardjo, and Joanna S.G. Slusky

2. Functional analysis and NMR studies of multi-drug efflux pumps / Dominic Ralph, VIvien Yeh, Alice Goode, Jessica M.A. Blair, Paul Williams, and Boyan B. Bonev

3. Using hydrogen/deuterium exchange mass spectrometry to understand bacterial membrane efflux proteins / Benjamin Russell Lewis, Dietmar Hammerschmid, Jakub Sys, and Eamonn Reading

4. Doxorubicin transport measurements for a bacterial multidrug transport protein / Pei-Yu Hsieh and Hendrik W. van Veen

5. Molecular investigation of drug efflux pumps by deep mutational scanning / Sujani Thavarasah, Gianmarco Meier, Anna Partas, and Markus A. Seeger

6. Reconstitution of tripartite efflux pumps MexAB-OprM and MacAB-TolC in biomimetic systems / Alexis Lodé, Marine Novelli, Céline Madigou, and Martin Picard

7. Preparation and activity characterization of a type IV ABC transporter efflux pump in peptidiscs / Julie Kerboeuf, Frédéric Galisson, Cécile Gonzalez, Jean-Michel Jault, and Elise Kaplan

8. Reconstitution and functional characterization of MmpL3, an essential transporter from Mycobacterium tuberculosis / Svitlana Babii, Komal Choukate, and Helen I. Zgurskaya

9. Discovery and functional characterization of archaeal efflux transporters / Thomas P. Thompson, Asma A. Fakhoury, Khondaker Miraz Rahman, and Brendan F. Gilmore

10. Crystallographic studies of efflux transporters with transport substrates and inhibitors / Satoshi Murakami

11. Computational approaches for modelling multidrug efflux pumps of the resistance nodulation-cell division superfamily / Mohd Athar, Silvia Gervasoni, Giuliano Malloci, Paolo Ruggerone, and Attilio V. Vargiu

12. MexB/inhibitor complexes preparation for crystallography and cryo-EM, comparison and possible pitfalls / Hilal Wehbi, Quentin Cece, Gilles Phan, and Isabelle Broutin

13. Stabilization of multidrug efflux transporter MdfA and antibody fragments complex by cross-linking for Cryo-EM single-particle analysis / Satomi Inaba-Inoue, Toshio Moriya, Norimichi Nomura, and Mikio Tanabe

14. Reconstitution of RND tripartite multidrug complexes for single-particle electron microscopy / Esther Boyer, Laetitia Daury, Marie-France Giraud, Marie-Christine Gerbod-Giannone, Sylvie Poussard, Isabelle Broutin, Jean-Christophe Taveau, and Olivier Lambert

15. Cry-electron microscopy (Cryo-EM) structural determination of the MmpL family of transporters / Emma Kundracik, William D. Gregor, Rakesh Maharjarn, Zhemin Zhang, Philip A. Klenotic, and Edward W. Yu

16. In situ structural analysis of tripartite efflux assemblies by cryoET / Xueting Zhou and Zhao Wang

17. Methods for investigating functional interplay between efflux pumps in Escherichia coli / Mallory Wright, Megan R. Caswell, and Georgina Cox

18. Quantitative single-cell imaging of efflux pump heterogeneity and antibiotic response dynamics / Ruizhe Li and Somenath Bakshi.

Notes:

Includes bibliographical references.

Other Format:

ebook version :

ISBN:

9780443429590

0443429596

OCLC:

1545080934

Publisher Number:

CIPO000290565