Protein termini. Part B / edited by Thomas Arnesen.

Format:

Book

Contributor:

Arnesen, Thomas, editor.

Series:

Methods in enzymology ; v. 719.

Methods in enzymology, 0076-6879 ; v. 719

Language:

English

Subjects (All):

Proteins--Synthesis.

Proteins.

Proteins--Analysis.

Enzymology.

protein.

Physical Description:

xx, 362 pages : illustrations (some color) ; 24 cm.

Edition:

First edition.

Place of Publication:

Cambridge, MA : Academic Press, an imprint of Elsevier, 2025.

Summary:

Protein termini represent a major route to protein regulation. From the moment the very first amino acid of a polypeptide chain exits the ribosome there is potential for steering from the cellular environment. This volume of Methods in Enzymology Modifications and Targeting of Protein Termini focuses on Protein N-termini and C-termini and their modifications which include acetylation, arginylation, myristoylation and oxidation. Also, the impact of terminal modifications is covered, in particular the impact on protein turnover and the ubiquitin E3 ligases which specifically recognize protein N-termini (N-degrons) and C-termini (C-degrons). In addition to the detailed methods and laboratory protocols, the chapters include informative overviews and reviews of the different subfields.

Contents:

Proteome analysis of puromycin-labeled nascent polypeptides / Koshi Imami

Hunter-Dia: An updated protocol for enrichment and mass spectrometry-based identification of protein N-terminal peptides / Raja Tamilselvan, Paula Dewes, Miguel Cosenza-Contreras, and Pitter F. Huesgen

TermineR: Bioinformatic processing of shotgun proteomics data for the annotation and quantitation of protein termini / Miguel Cosenza-Contreras, Pitter F. Huesgen, and Oliver Schilling - DEGRONOPEDIA: A practical guide to identifying and targeting protein degrons / Natalia A. Szulc and Wojciech Pokrzywa

Quantitative insights into protein turnover and ubiquitination with HiBiT and NanoBRET / Malgorzata Piechota and Wojciech Pokrzywa

Development of a flow cytometric method to evaluate the impact of N-terminal sequences on protein stability / Sabyasachi Sen, Khai Kee Kho, and Aditya M. Kunjapur

Generation and characterization of engineered N-degrons of the N-degron pathway using the ubiquitin-reference technique / Hye Yeon Kim, Tae Hyun Bae, Su Jin Lee, Su Bin Kim, Yong Tae Kwon, and Chang Hoon Ji

Characterization of the autophagic N-degron pathway and monitoring its chemical modulation for therapeutic development / Jihoon Lee, Gee Eun Lee, Chan Hoon Jung, Yoon Jee Lee, and Yong Tae Kwon

Characterization of the E3 ligase KCMF1 as a ZZ/N-recognin of the autophagic Arg/N-degron pathway / Ji Su Lee, Min Ju Lee, Su Bin Kim, Yong Tae Kwon, and Chang Hoon Ji

Identification of the Ac/N-degron recognition domain in the MARCHF6 E3 ubiquitin ligase / Jihye Yang and Cheol-Sang Hwang

Affinity purification-mass spectrometry to identify nuclear protein interactions of N-terminal acetyltransferase NAA40 / Ariel Klavaris, Ann-Christine König, Constantinos Demetriades, Stefanie M. Hauck, and Antonis Kirmizis

Proximity labelling of interacting proteins by TurboID / Greta Jarck and Nico Dissmeyer

Chemical proteomic approaches to investigate S-prenylation / Sarah Hassan, James Zhang, Woute Kallemeijn, and Edward W. Tate

Profiling of C-terminal prenylated proteins using tandem mass tagging / Harinarayanan Kottala, Yuanzhe Chen, and Mark D. Distefano

Fluorescence polarization binding assays for the E3 ligase FEM1C / Emma K. Seipp and Rong Huang.

Notes:

Includes bibliographical references.

ISBN:

9780443471926

0443471924

OCLC:

1524972998

Publisher Number:

CIPO000364975