Clostridioides difficile : methods and protocols / edited by Kurt H. Piepenbrink, Jennifer M. Auchtung

Format:

Book

Contributor:

Piepenbrink, Kurt H., editor.

Auchtung, Jennifer M., editor.

Series:

Springer protocols (Series)

Methods in molecular biology (Clifton, N.J.) ; v. 3046.

Methods in Molecular Biology, 1940-6029 ; 3046

Springer protocols

Language:

English

Subjects (All):

Clostridium difficile--Laboratory manuals.

Clostridium difficile.

Clostridioides difficile.

Medical Subjects:

Clostridioides difficile.

Physical Description:

1 online resource : illustrations

Place of Publication:

New York, NY : Humana Press, [2026]

Summary:

"This volume discusses the latest advancements on approaches used to characterize C. difficile physiology, including new methods for genetic modifications and novel models for studying host-pathogen interactions. The chapters in this book cover various topics such as FASTQ; CRISPR-AsCas12a and dAsCas 12a-mediated gene knockout and knockdown in Clostridioides difficile; preparation of spores with 70:30 method; measuring C. difficile adhesion to mucosal surfaces; and isolating C. difficile from animal samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Clostridioides difficile: Methods and Protocols is a valuable resource for researchers interested in learning more about this field. Gaining a deeper understanding of C. difficile physiology is crucial for developing new therapies for treatment and predicting the future evolution of this important clinical pathogen"-- Springer Nature Link

Contents:

From fastq to function to phylogeny : in silico methods for antimicrobial resistance and outbreak investigation / Mark D. Preston, Tsz Ting Chow, and Richard A. Stabler

Generating targeted mutants using a riboswitch to control plasmid behavior during allelic exchange mutagenesis / Joshua N. Brehm and Joseph A. Sorg

Use of the golden gate cloning technique for plasmid construction to generate conditional lethal mutants in C. difficile / Jeanne Malet-Villemagne, Marie-Emeline Zielinski, Assilina Parfut, and Thomas Candela

Crispr-AsCas12a and dAsCas12a-mediated gene knockout and knockdown in Clostridioides difficile / Duolong Zhu, Shaohui Wang, Xingmin Sun, and Robert A. Britton

Isolation and characterization of Clostridioides difficile bacteriophages / Alexia L. M. Royer, Andrew A. Umansky, and Louis-Charles Fortier

Preparation of spores with the 70:30 method / Eliane de Oliveira Ferreira

An integrated workflow for the purification and fluorescence imaging of Clostridioides difficile spores using lanthanide beta-diketone complexes / Ajitha Sundaresan and Ian Cheong

Nanoluciferase-based reporters for Clostridioides difficile / Jeroen Corver, Ana M. Oliveira Paiva, and Wiep Klaas Smits

Working with C. difficile in a hypoxic chamber / Léo C. Caulat, Aurélie Lotoux, Isabelle Martin-Verstraete, and Claire Morvan

Dual fluorescent labeling for bacterial aggregation assays / Anna M. Tingler, Erin C. Chard, Kate Psenka, and Melinda A. Engevik

In vitro measurement of Clostridioides difficile biofilm formation induced by gut and microbiota-derived signals / Carolina Altaf Figueiredo and Bruno Dupuy

Measuring C. difficile adhesion to mucosal surfaces / Ben S. Sidner, Hugh C. McCullough, Baishakhi Biswas, Jennifer M. Auchtung, and Kurt H. Piepenbrink

An in vitro model for studying interactions between gastrointestinal microbes and planktonic and sessile Clostridioides difficile populations / Jason Pizzini, Hugh C. McCullough, Ben S. Sidner, Robert A. Britton, Kurt H. Piepenbrink, and Jennifer M. Auchtung

Conventional, gnotobiotic, and humanized microbiota mouse models of C. difficile infection / Daniel Erickson and James Collins

Visualizing C. difficile during murine infection / Nicholas V. DiBenedetto and Aimee Shen

Clostridioides difficile infection in animals / Francisco A. Uzal

Isolating C. difficile from animal samples / Deiziane V. S. Costa and Cirle A. Warren

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed June 17, 2026)

Other Format:

Print version: Clostridioides difficile

ISBN:

9781071653289

1071653288

OCLC:

1591831062

Publisher Number:

CIPO000382327

Access Restriction:

Restricted for use by site license