Molecular Techniques.

Format:

Book

Author/Creator:

Chang, Ming-Mei, author.

Language:

English

Subjects (All):

Biology--Textbooks.

Biology.

Science--Textbooks.

Science.

Physical Description:

1 online resource

Place of Publication:

Geneseo, NY Milne Open Textbooks [2026]

Language Note:

In English.

Summary:

Molecular biology, examining the structures and functions of nucleic acids and proteins, is crucial to advancing biological science and improving human life, particularly in agriculture and medicine. Advances in the field can transform a crop to resist disease or uncover clues to fight illness by manipulating DNA. Techniques for molecular biology research and applications have advanced rapidly over the past decades, and undergraduates need hands-on experiences in this vital field of science. This text provides a structured approach to learning and practicing those techniques, covering DNA, RNA, and protein across five modules and is tailored for a one-semester undergraduate lab course. Each module extends 2-5 weeks of lab exercises and features.

Contents:

Introduction

To the Students

To the Instructors

Laboratory Safety

Pipetting

Making Solutions and Dilutions

Weekly Laboratory Notes

Laboratory Report

Module 1: Degenerate PCR and Topoisomerase (TOPO)-based DNA Cloning

Module 2: Analyzing Cloned NBS Sequences

Module 3: Gene Editing by Recombineering With or Without CRISPR-Cas9

Module 4: Studying Gene Expressions by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR)

Module 5: Exploring the Sensitivity and Specificity of Western Blot

Appendix A: Working Solutions for Immunodetection of Southern blot

Appendix B: General Remarks on Handling RNA

Appendix C: Instructor Key for Solutions and Dilutions

Notes:

Description based on online resource