Cellular spatial characterization and analysis / edited by Timothy Frankel, Arvind Rao

Format:

Book

Contributor:

Frankel, Timothy L., editor.

Rao, Arvind, editor.

Series:

Methods in molecular biology ; v. 2995.

Methods in molecular biology, 1940-6029 ; 2995

Language:

English

Subjects (All):

Bioinformatics.

Molecular biology--Technique.

Molecular biology.

Computational Biology.

Medical Subjects:

Computational Biology.

Physical Description:

1 online resource : illustrations (some color)

Place of Publication:

New York : Humana Press, [2026]

Summary:

"This volume covers the latest advancements in staining techniques, and protocols and process data using various bioinformatic approaches. The chapters in this book are divided into broad themes of traditional immunohistochemistry, advanced spatial proteomics, and spatial genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls"-- Springer Nature Link

Contents:

High‑plex tissue imaging with conventional immunofluorescence platforms and open‑source software via iterative bleaching extends multiplexity (IBEX) / Ian T. Linborn, Andrew Ionescu, Emily Spermane, Laurie M. Mattes, Ziv Yatin, Andrea J. Radke and Ronald N. Germain

Multiplex immunofluorescence staining of paraffin‑embedded tissue : coverslip mounting of tissue sections improves z‑axis optical resolution in widefield microscopy / Gitta A. Pedersen, Søren H. Elsborg, Rikke Nørregaard and Lene N. Nielsen

Evaluation of murine tissue architecture and cellular spatial relationships in murine tumor microenvironments utilizing multiplex fluorescent immunohistochemistry / Andrew Spiteri, Alberto Olivieri, Jake McGee, Aakshi Kumar, Rashi Kumar, Brian D. Griffith and Timothy L. Frankel

Autostaining immune multiplex immunohistochemistry panels and imaging analysis / Hidetoshi Mori and Alexander D. Borowsky

Fully integrated sample preparation‑based spatial proteomic analysis / Bojan Kosić, Xi Wang and Ruijin Tian

Detailed protocol for expansion of large tissue samples for expansion microscopy / Aleks B. Dam, Amalie M. Grønning, Benjamin B. Grønner, Frédéric H. Luginbühl and Lene N. Nielsen

Current methods in CODEX high‑multiplex microscopy to describe spatial relationships in the tissue microenvironment / Joseph Nichols, Amina Murmannova, Yannick Palmovski, Alexander Rochelvarg, Ahmad Matky, Mohammed Zidane, Annika Jäger, Jing Zhao, Yichao Zhang, Laurinda L. Mastrogiani, Karen Greer, Sriram Manoharan, Christine Beschorner and Christian M. Schürch

Mapping the tumor microenvironment with integrative single‑cell RNA sequencing and spatial proteomics : uncovering mechanisms of disease and therapeutic resistance / Ashley P. Tang, Ahmed M. Elhosny, Marina Pasca Di Magliano and Arvind Rao

Single‑molecule fluorescence in situ hybridization (smFISH) for RNA localization relative to DNA loci in bacteria / Juan Echeverry, Yu‑Huan Wang and Sangjin Kim

A protocol for spatial transcriptomics using the 10x Genomics Visium platform / Jing Yi Lee, Bansi Kannan, Zhen Wen Neo, Vikneswari Rajasegaran, Jeffrey Chum Tim, Joe Poh Sheng Teong and Jason Kronstad Chan

Highly multiplexed single‑cell in situ RNA and DNA analysis by consecutive hybridization / Lu Xia and Jia Guo

From raw data to biological insights : a practical guide for spatial transcriptomics analysis in R and Python / Ahmed M. Elhosny, Reza Kulkarni, Arvind Rao and Timothy L. Frankel

Notes:

Includes bibliographical references and index

Online resource; title from PDF title page (Springer Nature Link, viewed May 5, 2026)

Other Format:

Print version: Cellular spatial characterization and analysis

ISBN:

9781071650271

1071650270

OCLC:

1579845635

Publisher Number:

CIPO000353324

Access Restriction:

Restricted for use by site license